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Literature DB >> 8483906

Biochemical interaction of the Escherichia coli RecF, RecO, and RecR proteins with RecA protein and single-stranded DNA binding protein.

Abstract

The Escherichia coli RecF, RecO, and RecR proteins were analyzed for their effect on RecA-mediated pairing of single-stranded circular DNA and homologous linear duplex DNA substrates. As shown by other workers, joint molecule formation by RecA was inhibited by E. coli single-stranded DNA binding protein (SSB) when it was added to single-stranded DNA before RecA. This inhibitory effect was overcome by the addition of RecO and RecR or RecF, RecO, and RecR. Both the rate and extent of joint molecule formation were restored to the maximal level observed when SSB was added after RecA. RecF, RecO, and RecR proteins had no effect on the conversion of joint molecules to final products and only appeared to stimulate an early step in the pairing reaction. The stimulatory effect of RecF, RecO, and RecR was not seen without SSB or when SSB was added after RecA. RecF protein by itself inhibited reactions in mixtures containing RecA and SSB, and this inhibition was overcome by the addition of RecO and RecR. These data suggest that RecO and RecR, and possibly RecF, help RecA overcome inhibition by SSB and utilize SSB-single-stranded-DNA complexes as substrates.

Entities: Gene Species

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Year: 1993 PMID： 8483906 PMCID： PMC46408 DOI： 10.1073/pnas.90.9.3875

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

33 in total

1. Formation of recombinant lacZ+ DNA in conjugational crosses with a recB mutant of Escherichia coli K12 depends on recF, recJ, and recO.

Authors: R G Lloyd; N P Evans; C Buckman
Journal: Mol Gen Genet Date: 1987-08

2. The codon Adaptation Index--a measure of directional synonymous codon usage bias, and its potential applications.

Authors: P M Sharp; W H Li
Journal: Nucleic Acids Res Date: 1987-02-11 Impact factor: 16.971

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Journal: Annu Rev Biochem Date: 1987 Impact factor: 23.643

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Authors: G R Smith
Journal: Microbiol Rev Date: 1988-03

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Authors: P L Moreau
Journal: J Bacteriol Date: 1988-06 Impact factor: 3.490

6. Mechanism of the concerted action of recA protein and helix-destabilizing proteins in homologous recombination.

Authors: K Muniyappa; S L Shaner; S S Tsang; C M Radding
Journal: Proc Natl Acad Sci U S A Date: 1984-05 Impact factor: 11.205

7. Intermediates in homologous pairing promoted by recA protein. Isolation and characterization of active presynaptic complexes.

Authors: S S Tsang; K Muniyappa; E Azhderian; D K Gonda; C M Radding; J Flory; J W Chase
Journal: J Mol Biol Date: 1985-09-20 Impact factor: 5.469

8. Effects of the Escherichia coli SSB protein on the binding of Escherichia coli RecA protein to single-stranded DNA. Demonstration of competitive binding and the lack of a specific protein-protein interaction.

Authors: S C Kowalczykowski; J Clow; R Somani; A Varghese
Journal: J Mol Biol Date: 1987-01-05 Impact factor: 5.469

9. Effects of Escherichia coli SSB protein on the single-stranded DNA-dependent ATPase activity of Escherichia coli RecA protein. Evidence that SSB protein facilitates the binding of RecA protein to regions of secondary structure within single-stranded DNA.

Authors: S C Kowalczykowski; R A Krupp
Journal: J Mol Biol Date: 1987-01-05 Impact factor: 5.469

10. Genetic recombination of bacterial plasmid DNA: effect of RecF pathway mutations on plasmid recombination in Escherichia coli.

Authors: R Kolodner; R A Fishel; M Howard
Journal: J Bacteriol Date: 1985-09 Impact factor: 3.490

113 in total

1. Impairment of lagging strand synthesis triggers the formation of a RuvABC substrate at replication forks.

Authors: M J Flores; H Bierne; S D Ehrlich; B Michel
Journal: EMBO J Date: 2001-02-01 Impact factor: 11.598

2. Assembly of the Escherichia coli RuvABC resolvasome directs the orientation of holliday junction resolution.

Authors: A J van Gool; N M Hajibagheri; A Stasiak; S C West
Journal: Genes Dev Date: 1999-07-15 Impact factor: 11.361

3. The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation.

Authors: J J Churchill; D G Anderson; S C Kowalczykowski
Journal: Genes Dev Date: 1999-04-01 Impact factor: 11.361

10. The phage lambda orf gene encodes a trans-acting factor that suppresses Escherichia coli recO, recR, and recF mutations for recombination of lambda but not of E. coli.

Authors: J A Sawitzke; F W Stahl
Journal: J Bacteriol Date: 1994-11 Impact factor: 3.490