Literature DB >> 8483445

Analysis of single protoplasts and regenerated plants by PCR and RAPD technology.

P T Brown1, F D Lange, E Kranz, H Lörz.   

Abstract

We investigated the use of the polymerase chain reaction (PCR) and the associated random amplification of polymorphic DNA (RAPD) technique in the analysis of DNA and specific genes in plant cells at different stages of regeneration in in vitro cultures. We demonstrate that both procedures can be used to differentiate reproducibly between closely related species as well as to reveal levels of DNA polymorphism in regenerated plants. We also demonstrate that both procedures, using protocols that we have developed, are applicable at all tissue culture stages, from single isolated protoplasts to regenerated plants. Possible explanations for the variation levels detected in regenerated wheat plants are advanced.

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Year:  1993        PMID： 8483445     DOI： 10.1007/bf00279433

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  14 in total

1.  Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors:  E M Southern
Journal:  J Mol Biol       Date:  1975-11-05       Impact factor: 5.469

2.  Somaclonal variation - a novel source of variability from cell cultures for plant improvement.

Authors:  P J Larkin; W R Scowcroft
Journal:  Theor Appl Genet       Date:  1981-10       Impact factor: 5.699

3.  Parentage determination in maize hybrids using the arbitrarily primed polymerase chain reaction (AP-PCR).

Authors:  J Welsh; R J Honeycutt; M McClelland; B W Sobral
Journal:  Theor Appl Genet       Date:  1991-07       Impact factor: 5.699

4.  Identification of broccoli and cauliflower cultivars with RAPD markers.

Authors:  J Hu; C F Quiros
Journal:  Plant Cell Rep       Date:  1991-12       Impact factor: 4.570

5.  DNA polymorphisms amplified by arbitrary primers are useful as genetic markers.

Authors:  J G Williams; A R Kubelik; K J Livak; J A Rafalski; S V Tingey
Journal:  Nucleic Acids Res       Date:  1990-11-25       Impact factor: 16.971

6.  Amplification and analysis of DNA sequences in single human sperm and diploid cells.

Authors:  H H Li; U B Gyllensten; X F Cui; R K Saiki; H A Erlich; N Arnheim
Journal:  Nature       Date:  1988-09-29       Impact factor: 49.962

7.  Rapid identification of markers linked to a Pseudomonas resistance gene in tomato by using random primers and near-isogenic lines.

Authors:  G B Martin; J G Williams; S D Tanksley
Journal:  Proc Natl Acad Sci U S A       Date:  1991-03-15       Impact factor: 11.205

8.  Fingerprinting genomes using PCR with arbitrary primers.

Authors:  J Welsh; M McClelland
Journal:  Nucleic Acids Res       Date:  1990-12-25       Impact factor: 16.971

9.  Hybrid genes in the analysis of transformation conditions : I. Setting up a simple method for direct gene transfer in plant protoplasts.

Authors:  I Negrutiu; R Shillito; I Potrykus; G Biasini; F Sala
Journal:  Plant Mol Biol       Date:  1987-09       Impact factor: 4.076

10.  Stable transformation of barley via PEG-induced direct DNA uptake into protoplasts.

Authors:  P A Lazzeri; R Brettschneider; R Lührs; H Lörz
Journal:  Theor Appl Genet       Date:  1991-04       Impact factor: 5.699
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  11 in total

1.  Genomic instability in phenotypically normal regenerants of medicinal plant Codonopsis lanceolata Benth. et Hook. f., as revealed by ISSR and RAPD markers.

Authors:  W L Guo; L Gong; Z F Ding; Y D Li; F X Li; S P Zhao; B Liu
Journal:  Plant Cell Rep       Date:  2006-03-31       Impact factor: 4.570

2.  The detection of somaclonal variants of beet using RAPD.

Authors:  M T Munthali; H J Newbury; B V Ford-Lloyd
Journal:  Plant Cell Rep       Date:  1996-03       Impact factor: 4.570

3.  Identification of chrysanthemum cultivars and stability of DNA fingerprint patterns.

Authors:  K Wolff; E Zietkiewicz; H Hofstra
Journal:  Theor Appl Genet       Date:  1995-08       Impact factor: 5.699

4.  Use of random amplified polymorphic DNA (RAPD) markers in the discrimination and verification of genotypes in Eucalyptus.

Authors:  M Keil; A R Griffin
Journal:  Theor Appl Genet       Date:  1994-10       Impact factor: 5.699

5.  MAAP: a versatile and universal tool for genome analysis.

Authors:  G Caetano-Anollés
Journal:  Plant Mol Biol       Date:  1994-09       Impact factor: 4.076

6.  Evidence for genomic changes in transgenic rice (Oryza sativa L.) recovered from protoplasts.

Authors:  P H Bao; S Granata; S Castiglione; G Wang; C Giordani; E Cuzzoni; G Damiani; C Bandi; S K Datta; K Datta; I Potrykus; A Callegarin; F Sala
Journal:  Transgenic Res       Date:  1996-03       Impact factor: 2.788

7.  RAPD polymorphisms among variant and phenotypically normal rice (Oryza sativa var.indica) somaclonal progenies.

Authors:  I D Godwin; N Sangduen; R Kunanuvatchaidach; G Piperidis; S W Adkins
Journal:  Plant Cell Rep       Date:  1997-02       Impact factor: 4.570

8.  Heterologous expression of Arabidopsis ABF4 gene in potato enhances tuberization through ABA-GA crosstalk regulation.

Authors:  María Noelia Muñiz García; Margarita Stritzler; Daniela Andrea Capiati
Journal:  Planta       Date:  2013-11-28       Impact factor: 4.116

9.  Rapid plant regeneration and analysis of genetic fidelity of in vitro derived plants of Chlorophytum arundinaceum Baker--an endangered medicinal herb.

Authors:  S K Lattoo; S Bamotra; R Sapru Dhar; S Khan; A K Dhar
Journal:  Plant Cell Rep       Date:  2006-02-11       Impact factor: 4.570

10.  Gynogenic lines of onion (Allium cepa L.): evidence of their homozygosity.

Authors:  B Campion; B Bohanec; B Javornik
Journal:  Theor Appl Genet       Date:  1995-09       Impact factor: 5.699
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