Literature DB >> 8481007

High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes.

C Chang1, Y M Yu, S M Dai, S K Law, S S Gill.   

Abstract

Interactions among the 20-kDa protein gene and the cytA and cryIVD genes located in a 9.4-kb HindIII fragment were studied. A series of plasmids containing a combination of these different genes was constructed by using the Escherichia coli/Bacillus thuringiensis shuttle vector pHT3101. The plasmids were then used to transform an acrystalliferous strain, cryB, derived from B. thuringiensis subsp. kurstaki. The results from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblot analyses suggest that although the 20-kDa protein is required for the efficient CytA protein production in E. coli, it is not required in B. thuringiensis. With or without the truncated 20-kDa protein gene, the CtyA and/or CryIVD proteins are produced and form parasporal inclusions in B. thuringiensis cells. However, more-efficient expression is obtained when a second protein, probably acting as a chaperonin, is present. In addition, the time course studies show that the CytA and CryIVD proteins are coordinately produced. Both the crude B. thuringiensis culture and purified inclusions from each recombinant B. thuringiensis strain are toxic to Culex quinquefasciatus larvae. The parasporal inclusions formed in B. thuringiensis cells are mosquitocidal, with CytA synergizing CryIVD toxicity.

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Year:  1993        PMID: 8481007      PMCID: PMC202194          DOI: 10.1128/aem.59.3.815-821.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN: 0099-2240            Impact factor:   4.792


  31 in total

1.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

2.  Stabilization of translationally active mRNA by prokaryotic REP sequences.

Authors:  S F Newbury; N H Smith; E C Robinson; I D Hiles; C F Higgins
Journal:  Cell       Date:  1987-01-30       Impact factor: 41.582

3.  Identification of a positive retroregulator that stabilizes mRNAs in bacteria.

Authors:  H C Wong; S Chang
Journal:  Proc Natl Acad Sci U S A       Date:  1986-05       Impact factor: 11.205

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Isolation of a relatively nontoxic 65-kilodalton protein inclusion from the parasporal body of Bacillus thuringiensis subsp. israelensis.

Authors:  J E Ibarra; B A Federici
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

6.  Bacillus thuringiensis var. israelensis delta-endotoxin. Cloning and expression of the toxin in sporogenic and asporogenic strains of Bacillus subtilis.

Authors:  E S Ward; A R Ridley; D J Ellar; J A Todd
Journal:  J Mol Biol       Date:  1986-09-05       Impact factor: 5.469

7.  Delta endotoxin of Bacillus thuringiensis subsp. israelensis.

Authors:  J L Armstrong; G F Rohrmann; G S Beaudreau
Journal:  J Bacteriol       Date:  1985-01       Impact factor: 3.490

8.  Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis.

Authors:  K M McLean; H R Whiteley
Journal:  J Bacteriol       Date:  1987-03       Impact factor: 3.490

9.  Cytolytic activity and immunological similarity of the Bacillus thuringiensis subsp. israelensis and Bacillus thuringiensis subsp. morrisoni isolate PG-14 toxins.

Authors:  S S Gill; J M Hornung; J E Ibarra; G J Singh; B A Federici
Journal:  Appl Environ Microbiol       Date:  1987-06       Impact factor: 4.792

10.  Cell membrane interaction of Bacillus thuringiensis subsp. israelensis cytolytic toxins.

Authors:  S S Gill; G J Singh; J M Hornung
Journal:  Infect Immun       Date:  1987-05       Impact factor: 3.441

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  52 in total

Review 1.  Cytolytic toxin Cyt1A and its mechanism of membrane damage: data and hypotheses.

Authors:  Peter Butko
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

2.  Aedes aegypti alkaline phosphatase ALP1 is a functional receptor of Bacillus thuringiensis Cry4Ba and Cry11Aa toxins.

Authors:  Alan I Jiménez; Esmeralda Z Reyes; Angeles Cancino-Rodezno; Leidy P Bedoya-Pérez; Gustavo G Caballero-Flores; Luis F Muriel-Millan; Supaporn Likitvivatanavong; Sarjeet S Gill; Alejandra Bravo; Mario Soberón
Journal:  Insect Biochem Mol Biol       Date:  2012-06-20       Impact factor: 4.714

3.  Cadherin, alkaline phosphatase, and aminopeptidase N as receptors of Cry11Ba toxin from Bacillus thuringiensis subsp. jegathesan in Aedes aegypti.

Authors:  Supaporn Likitvivatanavong; Jianwu Chen; Alejandra Bravo; Mario Soberón; Sarjeet S Gill
Journal:  Appl Environ Microbiol       Date:  2010-10-29       Impact factor: 4.792

4.  Bacillus thuringiensis subsp. israelensis Cyt1Aa synergizes Cry11Aa toxin by functioning as a membrane-bound receptor.

Authors:  Claudia Pérez; Luisa E Fernandez; Jianguang Sun; Jorge Luis Folch; Sarjeet S Gill; Mario Soberón; Alejandra Bravo
Journal:  Proc Natl Acad Sci U S A       Date:  2005-12-09       Impact factor: 11.205

5.  Bacillus thuringiensis ssp. israelensis Cyt1Aa enhances activity of Cry11Aa toxin by facilitating the formation of a pre-pore oligomeric structure.

Authors:  Claudia Pérez; Carlos Muñoz-Garay; Leivi C Portugal; Jorge Sánchez; Sarjeet S Gill; Mario Soberón; Alejandra Bravo
Journal:  Cell Microbiol       Date:  2007-08-02       Impact factor: 3.715

6.  Distribution and diversity of Dipteran-specific cry and cyt genes in native Bacillus thuringiensis strains obtained from different ecosystems of Iran.

Authors:  Gholamreza Salehi Jouzani; Ali Pourjan Abad; Ali Seifinejad; Rasoul Marzban; Khalil Kariman; Bahram Maleki
Journal:  J Ind Microbiol Biotechnol       Date:  2007-11-13       Impact factor: 3.346

7.  Oligomerization of Cry11Aa from Bacillus thuringiensis has an important role in toxicity against Aedes aegypti.

Authors:  Carlos Muñoz-Garay; Claudia Rodríguez-Almazán; Jose N Aguilar; Leivi Portugal; Isabel Gómez; Gloria Saab-Rincon; Mario Soberón; Alejandra Bravo
Journal:  Appl Environ Microbiol       Date:  2009-10-09       Impact factor: 4.792

Review 8.  Bacillus thuringiensis and its pesticidal crystal proteins.

Authors:  E Schnepf; N Crickmore; J Van Rie; D Lereclus; J Baum; J Feitelson; D R Zeigler; D H Dean
Journal:  Microbiol Mol Biol Rev       Date:  1998-09       Impact factor: 11.056

9.  An alpha-amylase is a novel receptor for Bacillus thuringiensis ssp. israelensis Cry4Ba and Cry11Aa toxins in the malaria vector mosquito Anopheles albimanus (Diptera: Culicidae).

Authors:  Maria Teresa Fernandez-Luna; Humberto Lanz-Mendoza; Sarjeet S Gill; Alejandra Bravo; Mario Soberon; Juan Miranda-Rios
Journal:  Environ Microbiol       Date:  2009-12-04       Impact factor: 5.491

10.  Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  E Ben-Dov; S Boussiba; A Zaritsky
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

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