Literature DB >> 3546262

Expression in Escherichia coli of a cloned crystal protein gene of Bacillus thuringiensis subsp. israelensis.

K M McLean, H R Whiteley.   

Abstract

A ca. 10-kilobase (kb) HindIII fragment of plasmid DNA from Bacillus thuringiensis subsp. israelensis was cloned into plasmid pUC9 and transformed into Escherichia coli. Extracts of the recombinant strain contained a 27-kilodalton (kDa) peptide that reacted with antibodies to a 27-kDa peptide isolated from crystals produced by B. thuringiensis subsp. israelensis. Extracts of the recombinant strain were hemolytic and toxic to Aedes aegypti larvae. Full expression of the 27-kDa peptide required the presence of a ca. 0.8-kb region of DNA located 4 kb upstream from the structural gene; the 0.8-kb region could be present in cis or trans relative to the gene and apparently acted post-transcriptionally. Analysis of maxicells showed that the 10-kb insert also coded for peptides of 67, 20, and 16 kDa; data obtained with different subclones suggest that the 20-kDa peptide is encoded in the 0.8-kb DNA region.

Entities:  

Mesh：

Substances：

Year:  1987        PMID： 3546262      PMCID： PMC211895          DOI： 10.1128/jb.169.3.1017-1023.1987

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  28 in total

1.  Composition and Toxicity of the Inclusion of Bacillus thuringiensis subsp. israelensis.

Authors:  J P Insell; P C Fitz-James
Journal:  Appl Environ Microbiol       Date:  1985-07       Impact factor: 4.792

2.  Structural similarity between the lepidoptera- and diptera-specific insecticidal endotoxin genes of Bacillus thuringiensis subsp. "kurstaki" and "israelensis".

Authors:  L Thorne; F Garduno; T Thompson; D Decker; M Zounes; M Wild; A M Walfield; T J Pollock
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

3.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

4.  Isolation of a relatively nontoxic 65-kilodalton protein inclusion from the parasporal body of Bacillus thuringiensis subsp. israelensis.

Authors:  J E Ibarra; B A Federici
Journal:  J Bacteriol       Date:  1986-02       Impact factor: 3.490

5.  Diversity of locations for Bacillus thuringiensis crystal protein genes.

Authors:  J W Kronstad; H E Schnepf; H R Whiteley
Journal:  J Bacteriol       Date:  1983-04       Impact factor: 3.490

6.  [Electron microscope study of "Bacillus thuringiensis" var. "Israelensis" sporulation and crystal biogenesis (author's transl)].

Authors:  J F Charles; H de Barjac
Journal:  Ann Microbiol (Paris)       Date:  1982 May-Jun

7.  The isolation of structural genes from libraries of eucaryotic DNA.

Authors:  T Maniatis; R C Hardison; E Lacy; J Lauer; C O'Connell; D Quon; G K Sim; A Efstratiadis
Journal:  Cell       Date:  1978-10       Impact factor: 41.582

8.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

9.  Cloning and expression in Escherichia coli of the insecticidal delta-endotoxin gene of Bacillus thuringiensis var. israelensis.

Authors:  E S Ward; D J Ellar; J A Todd
Journal:  FEBS Lett       Date:  1984-10-01       Impact factor: 4.124

10.  Molecular cloning and the nucleotide sequence of the Mr 28 000 crystal protein gene of Bacillus thuringiensis subsp. israelensis.

Authors:  C Waalwijk; A M Dullemans; M E van Workum; B Visser
Journal:  Nucleic Acids Res       Date:  1985-11-25       Impact factor: 16.971
View more
  30 in total

1.  Overproduction of delta-endotoxins by sporeless Bacillus thuringiensis mutants obtained by nitrous acid mutagenesis.

Authors:  Saoussen Ben Khedher; Nabil Zouari; Nadia Messaddeq; Patrick Schultz; Samir Jaoua
Journal:  Curr Microbiol       Date:  2010-05-20       Impact factor: 2.188

2.  Cloning and expression of two homologous genes of Bacillus thuringiensis subsp. israelensis which encode 130-kilodalton mosquitocidal proteins.

Authors:  E S Ward; D J Ellar
Journal:  J Bacteriol       Date:  1988-02       Impact factor: 3.490

3.  The glycoprotein toxin of Bacillus thuringiensis subsp. israelensis indicates a lectinlike receptor in the larval mosquito gut.

Authors:  G Muthukumar; K W Nickerson
Journal:  Appl Environ Microbiol       Date:  1987-11       Impact factor: 4.792

4.  Molecular characterization of two novel crystal protein genes from Bacillus thuringiensis subsp. thompsoni.

Authors:  K L Brown; H R Whiteley
Journal:  J Bacteriol       Date:  1992-01       Impact factor: 3.490

5.  Isolation of the second Bacillus thuringiensis RNA polymerase that transcribes from a crystal protein gene promoter.

Authors:  K L Brown; H R Whiteley
Journal:  J Bacteriol       Date:  1990-12       Impact factor: 3.490

6.  Expression of cryIVA and cryIVB Genes, Independently or in Combination, in a Crystal-Negative Strain of Bacillus thuringiensis subsp. israelensis.

Authors:  A Delécluse; S Poncet; A Klier; G Rapoport
Journal:  Appl Environ Microbiol       Date:  1993-11       Impact factor: 4.792

7.  High-level cryIVD and cytA gene expression in Bacillus thuringiensis does not require the 20-kilodalton protein, and the coexpressed gene products are synergistic in their toxicity to mosquitoes.

Authors:  C Chang; Y M Yu; S M Dai; S K Law; S S Gill
Journal:  Appl Environ Microbiol       Date:  1993-03       Impact factor: 4.792

8.  Mosquito larvicidal activity of Escherichia coli with combinations of genes from Bacillus thuringiensis subsp. israelensis.

Authors:  E Ben-Dov; S Boussiba; A Zaritsky
Journal:  J Bacteriol       Date:  1995-05       Impact factor: 3.490

9.  Expression of the cryIB crystal protein gene of Bacillus thuringiensis.

Authors:  B L Brizzard; H E Schnepf; J W Kronstad
Journal:  Mol Gen Genet       Date:  1991-12

Review 10.  Insecticidal crystal proteins of Bacillus thuringiensis.

Authors:  H Höfte; H R Whiteley
Journal:  Microbiol Rev       Date:  1989-06
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.