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Literature DB >> 8481004

ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes.

R Davis¹, D van der Lelie, A Mercenier, C Daly, G F Fitzgerald.

Abstract

Two genes from the total genomic DNA of dairy starter culture Lactococcus lactis subsp. cremoris UC503, encoding ScrFI modification enzymes, have been cloned and expressed in Escherichia coli. No homology between the two methylase genes was detected, and inverse polymerase chain reaction of flanking chromosomal DNA indicated that both were linked on the Lactococcus genome. Neither clone encoded the cognate endonuclease. The DNA sequence of one of the methylase genes (encoded by pCI931M) was determined and consisted of an open reading frame 1,170 bp long, which could encode a protein of 389 amino acids (M(r), 44.5). The amino acid sequence contained the highly characteristic motifs of an m5C methylase. Extensive regions of homology were observed with the methylases of NlaX, EcoRII, and Dcm.

Entities: Gene Species

Mesh：

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Year: 1993 PMID： 8481004 PMCID： PMC202189 DOI： 10.1128/aem.59.3.777-785.1993

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

38 in total

1. In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage.

Authors: C Hill; L A Miller; T R Klaenhammer
Journal: J Bacteriol Date: 1991-07 Impact factor: 3.490

2. Restriction and modification in group N streptococci: effect of heat on development of modified lytic bacteriophage.

Authors: M E Sanders; T R Klaenhammer
Journal: Appl Environ Microbiol Date: 1980-09 Impact factor: 4.792

3. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

Authors: M Nelson; M McClelland
Journal: Nucleic Acids Res Date: 1989 Impact factor: 16.971

4. Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.

Authors: J M van der Vossen; D van der Lelie; G Venema
Journal: Appl Environ Microbiol Date: 1987-10 Impact factor: 4.792

5. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

Authors: H W Boyer; D Roulland-Dussoix
Journal: J Mol Biol Date: 1969-05-14 Impact factor: 5.469

6. New versatile cloning and sequencing vectors based on bacteriophage M13.

Authors: M P Kieny; R Lathe; J P Lecocq
Journal: Gene Date: 1983-12 Impact factor: 3.688

7. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

Authors: E A Raleigh; G Wilson
Journal: Proc Natl Acad Sci U S A Date: 1986-12 Impact factor: 11.205

8. Improved medium for lactic streptococci and their bacteriophages.

Authors: B E Terzaghi; W E Sandine
Journal: Appl Microbiol Date: 1975-06

9. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

Authors: M Noyer-Weidner; R Diaz; L Reiners
Journal: Mol Gen Genet Date: 1986-12

10. Kinetic and catalytic mechanism of HhaI methyltransferase.

Authors: J C Wu; D V Santi
Journal: J Biol Chem Date: 1987-04-05 Impact factor: 5.157

20 in total

1. Transcriptional analysis and regulation of expression of the ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503.

Authors: D Butler; G F Fitzgerald
Journal: J Bacteriol Date: 2001-08 Impact factor: 3.490

2. Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

Authors: Giedrius Vilkaitis; Arvydas Lubys; Egle Merkiene; Albertas Timinskas; Arvydas Janulaitis; Saulius Klimasauskas
Journal: Nucleic Acids Res Date: 2002-04-01 Impact factor: 16.971

3. Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56.

Authors: N R Nyengaard; J Falkenberg-Klok; J Josephsen
Journal: Appl Environ Microbiol Date: 1996-09 Impact factor: 4.792

ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes.

1. In vivo genetic exchange of a functional domain from a type II A methylase between lactococcal plasmid pTR2030 and a virulent bacteriophage.

2. Restriction and modification in group N streptococci: effect of heat on development of modified lytic bacteriophage.

3. Effect of site-specific methylation on DNA modification methyltransferases and restriction endonucleases.

4. Isolation and characterization of Streptococcus cremoris Wg2-specific promoters.

5. A complementation analysis of the restriction and modification of DNA in Escherichia coli.

6. New versatile cloning and sequencing vectors based on bacteriophage M13.

7. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.

8. Improved medium for lactic streptococci and their bacteriophages.

9. Cytosine-specific DNA modification interferes with plasmid establishment in Escherichia coli K12: involvement of rglB.

10. Kinetic and catalytic mechanism of HhaI methyltransferase.

1. Transcriptional analysis and regulation of expression of the ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503.

2. Circular permutation of DNA cytosine-N4 methyltransferases: in vivo coexistence in the BcnI system and in vitro probing by hybrid formation.

3. Cloning and analysis of the restriction-modification system LlaBI, a bacteriophage resistance system from Lactococcus lactis subsp. cremoris W56.

Review 4. Bacteriophage resistance in Lactococcus.

5. A type IC restriction-modification system in Lactococcus lactis.

6. DNA-methyltransferase SsoII interaction with own promoter region binding site.

Review 7. Biotechnology of lactic acid bacteria with special reference to bacteriophage resistance.

8. Effect of site-specific methylation on restriction endonucleases and DNA modification methyltransferases.

9. Determination of methylation specificity of DsaV methyltransferase by a simple biochemical method.

10. The plasmid complement of Lactococcus lactis UC509.9 encodes multiple bacteriophage resistance systems.