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Literature DB >> 6323254

New versatile cloning and sequencing vectors based on bacteriophage M13.

Abstract

A new pair of cloning and sequencing vectors based on bacteriophage M13mp7 has been developed. These vectors (M13tg130 and M13tg131) contain, in addition to the EcoRI, BamHI, HindIII, SmaI, SalI and PstI sites present in other vectors [cf., M13mp8 and M13mp9, Messing and Vieira, Gene 19 (1982) 269-276], unique restriction recognition sequences for the enzymes EcoRV, KpnI, SphI, SstI and XbaI. A restriction site for the enzyme BglII has been incorporated into the polylinker region of one of the vector pair to permit rapid discrimination between the two vectors.

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Year: 1983 PMID： 6323254 DOI： 10.1016/0378-1119(83)90039-2

Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688

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Cited

117 in total

1. Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.

Authors: J Yang; P Wang; A J Pittard
Journal: J Bacteriol Date: 1999-10 Impact factor: 3.490

2. Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid.

Authors: V Athanasopoulos; J Praszkier; A J Pittard
Journal: J Bacteriol Date: 1999-03 Impact factor: 3.490

New versatile cloning and sequencing vectors based on bacteriophage M13.

1. Mechanism of repression of the aroP P2 promoter by the TyrR protein of Escherichia coli.

2. Analysis of elements involved in pseudoknot-dependent expression and regulation of the repA gene of an IncL/M plasmid.

3. Patterns of sequence conservation in the S-Layer proteins and related sequences in Clostridium difficile.

4. Mutations affecting translational coupling between the rep genes of an IncB miniplasmid.

5. Pseudoknot-dependent translational coupling in repBA genes of the IncB plasmid pMU720 involves reinitiation.

6. Structure and expression of a light-inducible shoot-specific rice gene.

7. Nucleotide sequence of the osmoregulatory proU operon of Escherichia coli.

8. Interaction of the initiator protein of an IncB plasmid with its origin of DNA replication.

9. Regulation of aroL expression by TyrR protein and Trp repressor in Escherichia coli K-12.

10. Isolation and characterization of ropA homologous genes from Rhizobium leguminosarum biovars viciae and trifolii.