B S Minhas1. 1. Department of Obstetrics and Gynecology, East Tennessee State University, Johnson City.
Abstract
OBJECTIVE: The purpose of this study was to evaluate the effects of platelet-activating factor treatment on human spermatozoa fertilizing capability, as assessed by the zona-free hamster penetration assay. STUDY DESIGN: Twenty-three semen specimens were divided into two groups on the basis of initial motile sperm count greater or less than 20 x 10(6)/ml. Each sample served as its own treatment control. Twice-washed spermatozoa were treated with 1 x 10(-7) mol/L of platelet-activating factor before performing the sperm penetration assay. For the two groups the percent penetrations and penetration index were compared with the paired t test, and an independent t test was used to compare parameters between groups. RESULTS: Significant enhancement of both percent penetrations (p < 0.0001) and penetration index (p < 0.005) were noted on platelet-activating factor treatment in both groups. Enhancement of both indices were noted to be independent of initial motile sperm counts. CONCLUSION: Platelet-activating factor treatment of human spermatozoa may be of potential therapeutic value in certain cases of asthenospermia or fertilization failure in human in vitro fertilization.
OBJECTIVE: The purpose of this study was to evaluate the effects of platelet-activating factor treatment on human spermatozoa fertilizing capability, as assessed by the zona-free hamster penetration assay. STUDY DESIGN: Twenty-three semen specimens were divided into two groups on the basis of initial motile sperm count greater or less than 20 x 10(6)/ml. Each sample served as its own treatment control. Twice-washed spermatozoa were treated with 1 x 10(-7) mol/L of platelet-activating factor before performing the sperm penetration assay. For the two groups the percent penetrations and penetration index were compared with the paired t test, and an independent t test was used to compare parameters between groups. RESULTS: Significant enhancement of both percent penetrations (p < 0.0001) and penetration index (p < 0.005) were noted on platelet-activating factor treatment in both groups. Enhancement of both indices were noted to be independent of initial motile sperm counts. CONCLUSION: Platelet-activating factor treatment of human spermatozoa may be of potential therapeutic value in certain cases of asthenospermia or fertilization failure in human in vitro fertilization.
Authors: Andrew A Toledo; Dorothy Mitchell-Leef; Carlene W Elsner; Scott M Slayden; William E Roudebush Journal: J Assist Reprod Genet Date: 2003-05 Impact factor: 3.412