Literature DB >> 8475095

Enzymatic modification of plasma low density lipoproteins in rabbits: a potential treatment for hypercholesterolemia.

R Labeque1, C J Mullon, J P Ferreira, R S Lees, R Langer.   

Abstract

Phospholipase A2 (EC 3.1.1.4) hydrolyzes certain phospholipids of low density lipoprotein (LDL). Plasma clearance of phospholipase A2-modified human LDL is up to 17 times faster than that of native human LDL in hypercholesterolemic rabbits. Modification of blood lipoproteins of hypercholesterolemic rabbits was performed by using an extracorporeal circuit containing immobilized phospholipase A2. After 90-min treatments, nearly 30% decreases in plasma cholesterol concentrations were observed. Erythrocyte, leukocyte, and platelet counts showed no net change after treatment. This technique does not require any fluid replacement or sorbent regeneration and offers a potential approach for lowering serum cholesterol and LDL levels.

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Year:  1993        PMID： 8475095      PMCID： PMC46323          DOI： 10.1073/pnas.90.8.3476

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  41 in total

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Journal:  J Biol Chem       Date:  1959-03       Impact factor: 5.157

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Journal:  N Engl J Med       Date:  1989-04-06       Impact factor: 91.245

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Authors:  Y Hachimori; M A Wells; D J Hanahan
Journal:  Biochemistry       Date:  1971-10-26       Impact factor: 3.162

5.  Comparison of selectivity of LDL removal by double filtration and dextran-sulfate cellulose column plasmapheresis, and changes of subfractionated plasma lipoproteins after plasmapheresis in heterozygous familial hypercholesterolemia.

Authors:  Y Homma; Y Mikami; H Tamachi; N Nakaya; H Nakamura; Y Goto
Journal:  Metabolism       Date:  1987-05       Impact factor: 8.694

6.  Enzymatic modification of low density lipoprotein by purified lipoxygenase plus phospholipase A2 mimics cell-mediated oxidative modification.

Authors:  C P Sparrow; S Parthasarathy; D Steinberg
Journal:  J Lipid Res       Date:  1988-06       Impact factor: 5.922

7.  Technetium-99m low density lipoproteins: preparation and biodistribution.

Authors:  R S Lees; H D Garabedian; A M Lees; D J Schumacher; A Miller; J L Isaacsohn; A Derksen; H W Strauss
Journal:  J Nucl Med       Date:  1985-09       Impact factor: 10.057

8.  Plasma-exchange therapy of homozygous familial hypercholesterolemia.

Authors:  M E King; J L Breslow; R S Lees
Journal:  N Engl J Med       Date:  1980-06-26       Impact factor: 91.245

9.  Turnover and tissue sites of degradation of glucosylated low density lipoprotein in normal and immunized rabbits.

Authors:  O Wiklund; J L Witztum; T E Carew; R C Pittman; R L Elam; D Steinberg
Journal:  J Lipid Res       Date:  1987-09       Impact factor: 5.922

10.  An enzymatic system for removing heparin in extracorporeal therapy.

Authors:  R Langer; R J Linhardt; S Hoffberg; A K Larsen; C L Cooney; D Tapper; M Klein
Journal:  Science       Date:  1982-07-16       Impact factor: 47.728
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  4 in total

1.  Increased hepatic cholesterol accumulation in transgenic mice overexpressing human secretory phospholipase A2 group IIA.

Authors:  Rolf Eckey; Mario Menschikowski; Peter Lattke; Werner Jaross
Journal:  Inflammation       Date:  2004-04       Impact factor: 4.092

2.  Secretory phospholipase A2, group IIA is a novel serum amyloid A target gene: activation of smooth muscle cell expression by an interleukin-1 receptor-independent mechanism.

Authors:  Christopher P Sullivan; Stephanie E Seidl; Celeste B Rich; Michel Raymondjean; Barbara M Schreiber
Journal:  J Biol Chem       Date:  2009-10-22       Impact factor: 5.157

3.  A study on the functional subunits of phospholipases A2 by enzyme immobilization.

Authors:  J P Ferreira; R Sasisekharan; O Louie; R Langer
Journal:  Biochem J       Date:  1994-10-15       Impact factor: 3.857

4.  Pancreatic carboxyl ester lipase: a circulating enzyme that modifies normal and oxidized lipoproteins in vitro.

Authors:  R Shamir; W J Johnson; K Morlock-Fitzpatrick; R Zolfaghari; L Li; E Mas; D Lombardo; D W Morel; E A Fisher
Journal:  J Clin Invest       Date:  1996-04-01       Impact factor: 14.808
  4 in total

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