Literature DB >> 8475074

The p53 protein is an unusually shaped tetramer that binds directly to DNA.

P N Friedman1, X Chen, J Bargonetti, C Prives.   

Abstract

We have analyzed the size and structure of native immunopurified human p53 protein. By using a combination of chemical crosslinking, gel filtration chromatography, and zonal velocity gradient centrifugation, we have determined that the predominant form of p53 in such preparations is a tetramer. The behavior of purified p53 in gels and sucrose gradients implies that the protein has an extended shape. Wild-type p53 has been shown to bind specifically to sites in cellular and viral DNA. We show in this study by Southwestern ligand blotting and by analysis of DNA-bound crosslinked p53 that p53 monomers, dimers, and tetramers can bind directly to DNA.

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Year:  1993        PMID： 8475074      PMCID： PMC46291          DOI： 10.1073/pnas.90.8.3319

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  29 in total

1.  Nuclear accumulation of p53 protein is mediated by several nuclear localization signals and plays a role in tumorigenesis.

Authors:  G Shaulsky; N Goldfinger; A Ben-Ze'ev; V Rotter
Journal:  Mol Cell Biol       Date:  1990-12       Impact factor: 4.272

2.  Definition of a consensus binding site for p53.

Authors:  W S el-Deiry; S E Kern; J A Pietenpol; K W Kinzler; B Vogelstein
Journal:  Nat Genet       Date:  1992-04       Impact factor: 38.330

3.  The p53 nuclear localisation signal is structurally linked to a p34cdc2 kinase motif.

Authors:  C Addison; J R Jenkins; H W Stürzbecher
Journal:  Oncogene       Date:  1990-03       Impact factor: 9.867

4.  Transcriptional activation by wild-type but not transforming mutants of the p53 anti-oncogene.

Authors:  L Raycroft; H Y Wu; G Lozano
Journal:  Science       Date:  1990-08-31       Impact factor: 47.728

Review 5.  Structural aspects of the p53 protein in relation to gene evolution.

Authors:  T Soussi; C Caron de Fromentel; P May
Journal:  Oncogene       Date:  1990-07       Impact factor: 9.867

6.  The murine p53 protein blocks replication of SV40 DNA in vitro by inhibiting the initiation functions of SV40 large T antigen.

Authors:  E H Wang; P N Friedman; C Prives
Journal:  Cell       Date:  1989-05-05       Impact factor: 41.582

7.  Oligomerization of oncoprotein p53.

Authors:  S Kraiss; A Quaiser; M Oren; M Montenarh
Journal:  J Virol       Date:  1988-12       Impact factor: 5.103

Review 8.  Protein folding and intracellular transport: evaluation of conformational changes in nascent exocytotic proteins.

Authors:  M J Gething; K McCammon; J Sambrook
Journal:  Methods Cell Biol       Date:  1989       Impact factor: 1.441

9.  SV40 T antigen binds specifically to a cellular 53 K protein in vitro.

Authors:  F McCormick; R Clark; E Harlow; R Tjian
Journal:  Nature       Date:  1981-07-02       Impact factor: 49.962

10.  Presence of a potent transcription activating sequence in the p53 protein.

Authors:  S Fields; S K Jang
Journal:  Science       Date:  1990-08-31       Impact factor: 47.728
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  93 in total

1.  A leucine-rich nuclear export signal in the p53 tetramerization domain: regulation of subcellular localization and p53 activity by NES masking.

Authors:  J M Stommel; N D Marchenko; G S Jimenez; U M Moll; T J Hope; G M Wahl
Journal:  EMBO J       Date:  1999-03-15       Impact factor: 11.598

2.  Targeting protein inactivation through an oligomerization chain reaction.

Authors:  Francesco Contegno; Mario Cioce; Pier Giuseppe Pelicci; Saverio Minucci
Journal:  Proc Natl Acad Sci U S A       Date:  2002-02-12       Impact factor: 11.205

3.  Benign clonal keratinocyte patches with p53 mutations show no genetic link to synchronous squamous cell precancer or cancer in human skin.

Authors:  Z P Ren; A Ahmadian; F Pontén; M Nistér; C Berg; J Lundeberg; M Uhlén; J Pontén
Journal:  Am J Pathol       Date:  1997-05       Impact factor: 4.307

4.  A perspective on mechanisms of protein tetramer formation.

Authors:  Evan T Powers; David L Powers
Journal:  Biophys J       Date:  2003-12       Impact factor: 4.033

5.  Differentiated embryo-chondrocyte expressed gene 1 regulates p53-dependent cell survival versus cell death through macrophage inhibitory cytokine-1.

Authors:  Yingjuan Qian; Yong-Sam Jung; Xinbin Chen
Journal:  Proc Natl Acad Sci U S A       Date:  2012-06-21       Impact factor: 11.205

6.  p53-induced DNA bending and twisting: p53 tetramer binds on the outer side of a DNA loop and increases DNA twisting.

Authors:  A K Nagaich; V B Zhurkin; S R Durell; R L Jernigan; E Appella; R E Harrington
Journal:  Proc Natl Acad Sci U S A       Date:  1999-03-02       Impact factor: 11.205

7.  Cryo-EM-On-a-Chip: Custom-Designed Substrates for the 3D Analysis of Macromolecules.

Authors:  Nick A Alden; A Cameron Varano; William J Dearnaley; Maria J Solares; William Y Luqiu; Yanping Liang; Zhi Sheng; Sarah M McDonald; John Damiano; Jennifer McConnell; Madeline J Dukes; Deborah F Kelly
Journal:  Small       Date:  2019-04-08       Impact factor: 13.281

8.  Dimerization-induced corepressor binding and relaxed DNA-binding specificity are critical for PML/RARA-induced immortalization.

Authors:  Jun Zhou; Laurent Pérès; Nicole Honoré; Rihab Nasr; Jun Zhu; Hugues de Thé
Journal:  Proc Natl Acad Sci U S A       Date:  2006-06-06       Impact factor: 11.205

9.  The DNA binding activity of p53 displays reaction-diffusion kinetics.

Authors:  Peter Hinow; Carl E Rogers; Christopher E Barbieri; Jennifer A Pietenpol; Anne K Kenworthy; Emmanuele DiBenedetto
Journal:  Biophys J       Date:  2006-04-07       Impact factor: 4.033

10.  Mutant p53 perturbs DNA replication checkpoint control through TopBP1 and Treslin.

Authors:  Kang Liu; Fang-Tsyr Lin; Joshua D Graves; Yu-Ju Lee; Weei-Chin Lin
Journal:  Proc Natl Acad Sci U S A       Date:  2017-04-24       Impact factor: 11.205
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