| Literature DB >> 847467 |
R H Scheller, T L Thomas, A S Lee, W H Klein, W D Niles, R J Britten, E H Davidson.
Abstract
Interspersed repetitive sequences were isolated from sea urchin DNA by renaturing to low Cot followed by treatment with nuclease SI. Synthetic Eco RI sites were ligated onto the repetitive sequence elements, which were then inserted at the Eco RI site of plasmid RSF2124 and cloned. The repetitive sequences can be excised from the plasmid with Eco RI for further study.Entities:
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Year: 1977 PMID: 847467 DOI: 10.1126/science.847467
Source DB: PubMed Journal: Science ISSN: 0036-8075 Impact factor: 47.728