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Literature DB >> 8468307

Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.

B V Taylor¹, J Toy, T L Sit, A L Bognar.

Abstract

The DNA sequence of the valS gene from Lactobacillus casei and the predicted amino acid sequence of its valyl-tRNA synthetase product have been determined. An open reading frame coding for a protein of 901 amino acids was found. A clone containing the intact L. casei valS gene functionally complemented the temperature-sensitive growth of the valS mutant strain 236c of Escherichia coli. The valS gene and the downstream folylpolyglutamate synthetase gene are transcribed in the same direction but are separated by a putative transcription terminator.

Entities: Chemical Species

Mesh：

Substances：
Valine-tRNA Ligase

Year: 1993 PMID： 8468307 PMCID： PMC204542 DOI： 10.1128/jb.175.8.2475-2478.1993

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

22 in total

1. Acetylornithinase of Escherichia coli: partial purification and some properties.

Authors: H J VOGEL; D M BONNER
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2. Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus.

Authors: N J Brand; A R Fersht
Journal: Gene Date: 1986 Impact factor: 3.688

3. Cloning of uvrA, lexC and ssb genes of Escherichia coli.

Authors: A Sancar; W D Rupp
Journal: Biochem Biophys Res Commun Date: 1979-09-12 Impact factor: 3.575

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

5. Identification of the uvrA gene product.

Authors: A Sancar; R P Wharton; S Seltzer; B M Kacinski; N D Clarke; W D Rupp
Journal: J Mol Biol Date: 1981-05-05 Impact factor: 5.469

6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors: J Vieira; J Messing
Journal: Gene Date: 1982-10 Impact factor: 3.688

7. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

Authors: A P Feinberg; B Vogelstein
Journal: Anal Biochem Date: 1983-07-01 Impact factor: 3.365

8. Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product.

Authors: A L Bognar; C Osborne; B Shane; S C Singer; R Ferone
Journal: J Biol Chem Date: 1985-05-10 Impact factor: 5.157

Cloning and sequence determination of the valS gene, encoding valyl-tRNA synthetase in Lactobacillus casei.

1. Acetylornithinase of Escherichia coli: partial purification and some properties.

2. Molecular cloning of the gene encoding the valyl-tRNA synthetase from Bacillus stearothermophilus.

3. Cloning of uvrA, lexC and ssb genes of Escherichia coli.

4. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

5. Identification of the uvrA gene product.

6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

7. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.

8. Folylpoly-gamma-glutamate synthetase-dihydrofolate synthetase. Cloning and high expression of the Escherichia coli folC gene and purification and properties of the gene product.

9. DNA sequencing with chain-terminating inhibitors.

10. Studies on transformation of Escherichia coli with plasmids.

1. X-prolyl dipeptidyl aminopeptidase gene (pepX) is part of the glnRA operon in Lactobacillus rhamnosus.

2. Structure and regulation of expression of the Bacillus subtilis valyl-tRNA synthetase gene.