Literature DB >> 8457346

Transgenic embryo yield is increased by a simple, inexpensive micropipet treatment.

K Gundersen1, T A Hanley, J P Merlie.   

Abstract

A method for the treatment of micropipet tips for injection of DNA into pronuclei of mouse embryos is described. The method requires only standard laboratory equipment, a magnetic stirrer and an inexpensive commercially available grinding powder. The method involves little extra time and no special skills. After introduction of the method, the time required for injections was reduced by about 30%, and the yield of surviving embryos was increased by 28%.

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Year:  1993        PMID： 8457346

Source DB:  PubMed          Journal:  Biotechniques        ISSN： 0736-6205            Impact factor:   1.993


  6 in total

1.  The effect of coating single- and double-stranded DNA with the recombinase A protein of Escherichia coli on transgene integration in mice.

Authors:  Jeffrey B Mason; Jeffrey G Najarian; Gary B Anderson; James D Murray; Elizabeth A Maga
Journal:  Transgenic Res       Date:  2006-09-07       Impact factor: 2.788

2.  Id-1 as a possible transcriptional mediator of muscle disuse atrophy.

Authors:  K Gundersen; J P Merlie
Journal:  Proc Natl Acad Sci U S A       Date:  1994-04-26       Impact factor: 11.205

3.  Overexpression of myogenin in muscles of transgenic mice: interaction with Id-1, negative crossregulation of myogenic factors, and induction of extrasynaptic acetylcholine receptor expression.

Authors:  K Gundersen; I Rabben; B J Klocke; J P Merlie
Journal:  Mol Cell Biol       Date:  1995-12       Impact factor: 4.272

4.  ARIA/HRG regulates AChR epsilon subunit gene expression at the neuromuscular synapse via activation of phosphatidylinositol 3-kinase and Ras/MAPK pathway.

Authors:  M G Tansey; G C Chu; J P Merlie
Journal:  J Cell Biol       Date:  1996-07       Impact factor: 10.539

5.  Myogenin induces a shift of enzyme activity from glycolytic to oxidative metabolism in muscles of transgenic mice.

Authors:  S M Hughes; M M Chi; O H Lowry; K Gundersen
Journal:  J Cell Biol       Date:  1999-05-03       Impact factor: 10.539

6.  Neural regulation of muscle acetylcholine receptor epsilon- and alpha-subunit gene promoters in transgenic mice.

Authors:  K Gundersen; J R Sanes; J P Merlie
Journal:  J Cell Biol       Date:  1993-12       Impact factor: 10.539
  6 in total

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