Literature DB >> 8441702

Efficient random mutagenesis method with adjustable mutation frequency by use of PCR and dITP.

J H Spee1, W M de Vos, O P Kuipers.   

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Year:  1993        PMID: 8441702      PMCID: PMC309199          DOI: 10.1093/nar/21.3.777

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN: 0305-1048            Impact factor:   16.971


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  4 in total

1.  Random mutagenesis of gene-sized DNA molecules by use of PCR with Taq DNA polymerase.

Authors:  Y H Zhou; X P Zhang; R H Ebright
Journal:  Nucleic Acids Res       Date:  1991-11-11       Impact factor: 16.971

2.  Identification and characterization of the lantibiotic nisin Z, a natural nisin variant.

Authors:  J W Mulders; I J Boerrigter; H S Rollema; R J Siezen; W M de Vos
Journal:  Eur J Biochem       Date:  1991-11-01

3.  Improved site-directed mutagenesis method using PCR.

Authors:  O P Kuipers; H J Boot; W M de Vos
Journal:  Nucleic Acids Res       Date:  1991-08-25       Impact factor: 16.971

4.  DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.

Authors:  M A Innis; K B Myambo; D H Gelfand; M A Brow
Journal:  Proc Natl Acad Sci U S A       Date:  1988-12       Impact factor: 11.205

  4 in total
  46 in total

1.  Changing the recognition specificity of a DNA-methyltransferase by in vitro evolution.

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2.  Unlocking hidden genomic sequence.

Authors:  Jonathan M Keith; Duncan A E Cochran; Gita H Lala; Peter Adams; Darryn Bryant; Keith R Mitchelson
Journal:  Nucleic Acids Res       Date:  2004-02-18       Impact factor: 16.971

3.  Sequence saturation mutagenesis (SeSaM): a novel method for directed evolution.

Authors:  Tuck Seng Wong; Kang Lan Tee; Berhard Hauer; Ulrich Schwaneberg
Journal:  Nucleic Acids Res       Date:  2004-02-10       Impact factor: 16.971

4.  A simple and reproducible method for directed evolution: combination of random mutation with dITP and DNA fragmentation with endonuclease V.

Authors:  Zun Wang; Hai-Yan Wang; Hong Feng
Journal:  Mol Biotechnol       Date:  2013-01       Impact factor: 2.695

5.  Modeling the effects of prl mutations on the Escherichia coli SecY complex.

Authors:  Margaret A Smith; William M Clemons; Cathrine J DeMars; Ann M Flower
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6.  The ubiquitin-like protein Smt3p is activated for conjugation to other proteins by an Aos1p/Uba2p heterodimer.

Authors:  E S Johnson; I Schwienhorst; R J Dohmen; G Blobel
Journal:  EMBO J       Date:  1997-09-15       Impact factor: 11.598

7.  14-3-3 proteins are required for maintenance of Raf-1 phosphorylation and kinase activity.

Authors:  J A Thorson; L W Yu; A L Hsu; N Y Shih; P R Graves; J W Tanner; P M Allen; H Piwnica-Worms; A S Shaw
Journal:  Mol Cell Biol       Date:  1998-09       Impact factor: 4.272

8.  Structural and functional characterization of a noncanonical nucleoside triphosphate pyrophosphatase from Thermotoga maritima.

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Journal:  Acta Crystallogr D Biol Crystallogr       Date:  2013-01-18

9.  The mutagenic properties of BrdUTP in a random mutagenesis process.

Authors:  Xiangdong Ma; Tao Ke; Peihong Mao; Xiang Jin; Lixin Ma; Guangyuan He
Journal:  Mol Biol Rep       Date:  2007-09-15       Impact factor: 2.316

10.  Ambiguous base pairing of the purine analogue 1-(2-deoxy-beta-D-ribofuranosyl)-imidazole-4-carboxamide during PCR.

Authors:  M Sala; V Pezo; S Pochet; S Wain-Hobson
Journal:  Nucleic Acids Res       Date:  1996-09-01       Impact factor: 16.971

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