Literature DB >> 8440732

Construction of Zn2+/Cd2+ hypersensitive cyanobacterial mutants lacking a functional metallothionein locus.

J S Turner1, A P Morby, B A Whitton, A Gupta, N J Robinson.   

Abstract

Eukaryotic metallothioneins (MTs) have been extensively studied, but the precise functions of most of these molecules are not yet fully understood. Prokaryotes are often more tractable for the analysis of gene function and we report here the generation of mutants of Synechococcus PCC 7942 (strain R2-PIM8) deficient in the MT locus, smt. Viability of these mutants, designated R2-PIM8 (smt), reveals that prokaryotic MT performs no "vital" role (such as donation of metals to metallo-proteins) in Synechococcus. R2-PIM8 (smt) has reduced (approximately 5-fold) tolerance to elevated Zn2+, with detectable hypersensitivity to Cd2+. Restoration of Zn2+ tolerance was used as a selectable marker to isolate recombinants derived from R2-PIM8(smt) after reintroduction of a linear DNA fragment containing an uninterrupted smt locus. These smt-complemented cells also exhibited restored Cd2+ tolerance. Hypersensitivity to Cu2+ was not detected in R2-PIM8(smt) indicating independence of Cu2+ resistance from smt mediated metal (Zn2+/Cd2+) tolerance.

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Year:  1993        PMID： 8440732

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  25 in total

1.  Letter to the Editor: Backbone NMR assignments of a cyanobacterial transcriptional factor, SmtB, that binds zinc ions.

Authors:  T Kosada; E Hayato Morita; A Miura; T Yamazaki; H Hayashi; Y Kyogoku
Journal:  J Biomol NMR       Date:  1999-06       Impact factor: 2.835

Review 2.  Ecological genomics of marine picocyanobacteria.

Authors:  D J Scanlan; M Ostrowski; S Mazard; A Dufresne; L Garczarek; W R Hess; A F Post; M Hagemann; I Paulsen; F Partensky
Journal:  Microbiol Mol Biol Rev       Date:  2009-06       Impact factor: 11.056

Review 3.  Bacterial metallothioneins: past, present, and questions for the future.

Authors:  Claudia A Blindauer
Journal:  J Biol Inorg Chem       Date:  2011-05-19       Impact factor: 3.358

4.  Zn2+-sensing by the cyanobacterial metallothionein repressor SmtB: different motifs mediate metal-induced protein-DNA dissociation.

Authors:  J S Turner; P D Glands; A C Samson; N J Robinson
Journal:  Nucleic Acids Res       Date:  1996-10-01       Impact factor: 16.971

5.  Advances in characterizing microbial community change and resistance upon exposure to lead contamination: Implications for ecological risk assessment.

Authors:  S Elizabeth George; Yongshan Wan
Journal:  Crit Rev Environ Sci Technol       Date:  2019-12-23       Impact factor: 12.561

6.  Metalloregulation of the cyanobacterial smt locus: identification of SmtB binding sites and direct interaction with metals.

Authors:  J L Erbe; K B Taylor; L M Hall
Journal:  Nucleic Acids Res       Date:  1995-07-11       Impact factor: 16.971

Review 7.  Bacterial resistance mechanisms for heavy metals of environmental concern.

Authors:  G Ji; S Silver
Journal:  J Ind Microbiol       Date:  1995-02

8.  An SmtB-like repressor from Synechocystis PCC 6803 regulates a zinc exporter.

Authors:  C Thelwell; N J Robinson; J S Turner-Cavet
Journal:  Proc Natl Acad Sci U S A       Date:  1998-09-01       Impact factor: 11.205

9.  Construction of Zn2+/Cd(2+)-tolerant cyanobacteria with a modified metallothionein divergon: further analysis of the function and regulation of smt.

Authors:  J S Turner; N J Robinson; A Gupta
Journal:  J Ind Microbiol       Date:  1995 Mar-Apr

10.  Cyanobacteria MT gene SmtA enhance zinc tolerance in Arabidopsis.

Authors:  Jing Xu; Yong-Sheng Tian; Ri-He Peng; Ai-Sheng Xiong; Bo Zhu; Xi-Lin Hou; Quan-Hong Yao
Journal:  Mol Biol Rep       Date:  2010-02       Impact factor: 2.316
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