Isolation of a somatic cell hybrid retaining the der(16)t(12;16)(q13;p11.2) from a myxoid liposarcoma cell line.

We developed cell line LIS-3/SV40 from a primary myxoid liposarcoma with the t(12;16)(q13;p11.2) and trisomy 8. Following fusion of LIS-3/SV40 to mouse A9 cells, we obtained hybrid LIS-3/SV40/A9-B4 which contained the der(16) but neither the der(12) nor some of the normal human chromosomes, including 12 and 16. Furthermore, microclone library ML12q1315 was constructed from microdissected fragments of chromosome bands 12q13-15 and its microclones were found to span the breakpoint in LIS-3/SV40.