Literature DB >> 8427877

Purification and characterization of two distinct lipases from Candida cylindracea.

L Rúa1, T Díaz-Mauriño, V M Fernández, C Otero, A Ballesteros.   

Abstract

We have purified and characterized two isoenzymes from a commercial lipase preparation of Candida cylindracea. The purification procedure includes ethanol precipitation and DEAE-Sephacel and Sephacryl HR 100 chromatographies. Lipase A and lipase B were purified 11-fold with a 5% and 21% recovery in activity, respectively. The enzymes have similar amino acid content, N-terminal sequence and molecular weight, but differ on neutral sugar content, hydrophobicity, presence of isoforms and stability to pH and temperature. They also show some differences in the substrate specificity.

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Year:  1993        PMID： 8427877     DOI： 10.1016/0304-4165(93)90134-t

Source DB:  PubMed          Journal:  Biochim Biophys Acta        ISSN： 0006-3002


  17 in total

1.  Analysis of the gene family encoding lipases in Candida rugosa by competitive reverse transcription-PCR.

Authors:  G C Lee; S J Tang; K H Sun; J F Shaw
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

2.  Sequence of the lid affects activity and specificity of Candida rugosa lipase isoenzymes.

Authors:  Stefania Brocca; Francesco Secundo; Mattia Ossola; Lilia Alberghina; Giacomo Carrea; Marina Lotti
Journal:  Protein Sci       Date:  2003-10       Impact factor: 6.725

3.  Characterization of an organic solvent-tolerant lipase from Haloarcula sp. G41 and its application for biodiesel production.

Authors:  Xin Li; Hui-Ying Yu
Journal:  Folia Microbiol (Praha)       Date:  2014-05-02       Impact factor: 2.099

4.  Stability studies and effect of the initial oleic acid concentration on lipase production by Candida rugosa.

Authors:  M A Gordillo; N Obradors; J L Montesinos; F Valero; J Lafuente; C Solà
Journal:  Appl Microbiol Biotechnol       Date:  1995-04       Impact factor: 4.813

5.  Purification and characterization of a Penicillium sp. lipase which discriminates against diglycerides.

Authors:  K Gulomova; E Ziomek; J D Schrag; K Davranov; M Cygler
Journal:  Lipids       Date:  1996-04       Impact factor: 1.880

6.  Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris.

Authors:  Guan-Chiun Lee; Li-Chiun Lee; Vasyl Sava; Jei-Fu Shaw
Journal:  Biochem J       Date:  2002-09-01       Impact factor: 3.857

7.  Contribution to the study of the alteration of lipase activity of Candida rugosa by ions and buffers.

Authors:  M J Hernáiz; M Rua; B Celda; P Medina; J V Sinisterra; J M Sánchez-Montero
Journal:  Appl Biochem Biotechnol       Date:  1994-03       Impact factor: 2.926

8.  Expression of an organic solvent stable lipase from Staphylococcus epidermidis AT2.

Authors:  Raja Noor Zaliha Raja Abd Rahman; Nor Hafizah Ahmad Kamarudin; Jalimah Yunus; Abu Bakar Salleh; Mahiran Basri
Journal:  Int J Mol Sci       Date:  2010-09-13       Impact factor: 5.923

9.  Localization of lipase genes on Candida rugosa chromosomes.

Authors:  S Brocca; R Grandori; D Breviario; M Lotti
Journal:  Curr Genet       Date:  1995-10       Impact factor: 3.886

10.  Influence of cultivation conditions on the production of a thermostable extracellular lipase from Amycolatopsis mediterranei DSM 43304.

Authors:  Dharmendra S Dheeman; Jesus M Frias; Gary T M Henehan
Journal:  J Ind Microbiol Biotechnol       Date:  2009-10-06       Impact factor: 3.346
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