Literature DB >> 8427628

Liquid chromatographic analysis of amino acids: using dimethylamino-azobenzenesulfonyl chloride and Hypersil ODS column to analyze the composition of apo B peptides.

C Y Yang1, T Yang, B K Yeh.   

Abstract

A reliable high-performance liquid chromatography (HPLC) method is described for the separation of dimethylamino-azobenzenesulfonyl-amino acid (DABS-AA). The separation is accomplished by reversed-phase chromatography on a Hypersil ODS column (4.6 x 150 mm) with a Hewlett-Packard liquid chromatography system. In addition to the developed sample and solvent preparation procedure, this precolumn modification method using dimethylaminoazobenzene sulfonyl chloride (DABS-CL) for amino acid analysis is proved reliable and sensitive. Five apolipoprotein B-100 tryptic peptides, two of them containing cysteine, were demonstrated as examples for the general application of this method in amino acid analysis. It is a useful method for analysis of cysteine- and cystine-containing peptide and, furthermore, for determination of sulfhydryl and disulfide linkages of proteins.

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Year:  1993        PMID： 8427628     DOI： 10.1007/bf01024908

Source DB:  PubMed          Journal:  J Protein Chem        ISSN： 0277-8033


  10 in total

1.  Chromatographic analysis of amino acids and primary amines with o-phthalaldehyde detection.

Authors:  J R Cronin; P E Hare
Journal:  Anal Biochem       Date:  1977-07       Impact factor: 3.365

2.  Liquid chromatographic determination of amino acids after gas-phase hydrolysis and derivatization with (dimethylamino)azobenzenesulfonyl chloride.

Authors:  R Knecht; J Y Chang
Journal:  Anal Chem       Date:  1986-10       Impact factor: 6.986

3.  Isolation and characterization of sulfhydryl and disulfide peptides of human apolipoprotein B-100.

Authors:  C Y Yang; T W Kim; S A Weng; B R Lee; M L Yang; A M Gotto
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

4.  High-sensitivity amino acid analysis: methodology for the determination of amino acid compositions with less than 100 picomoles of peptides.

Authors:  P Böhlen; R Schroeder
Journal:  Anal Biochem       Date:  1982-10       Impact factor: 3.365

5.  Chromophoric labeling of amino acids with 4-dimethylaminoazobenzene-4'-sulfonyl chloride.

Authors:  J K Lin; J Y Chang
Journal:  Anal Chem       Date:  1975-08       Impact factor: 6.986

6.  Amino acid analysis by reverse-phase high-performance liquid chromatography: precolumn derivatization with phenylisothiocyanate.

Authors:  R L Heinrikson; S C Meredith
Journal:  Anal Biochem       Date:  1984-01       Impact factor: 3.365

7.  A complete separation of dimethylaminoazobenzenesulphonyl-amino acids. Amino acid analysis with low nanogram amounts of polypeptide with dimethylaminoazobenzenesulphonyl chloride.

Authors:  J Y Chang; R Knecht; D G Braun
Journal:  Biochem J       Date:  1982-06-01       Impact factor: 3.857

8.  Structure and conformational analysis of lipid-associating peptides of apolipoprotein B-100 produced by trypsinolysis.

Authors:  C Y Yang; T W Kim; Q Pao; L Chan; R D Knapp; A M Gotto; H J Pownall
Journal:  J Protein Chem       Date:  1989-12

9.  O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

Authors:  J R Benson; P E Hare
Journal:  Proc Natl Acad Sci U S A       Date:  1975-02       Impact factor: 11.205

10.  Separation of phenylthiocarbamyl amino acids by high-performance liquid chromatography on Spherisob octadecylsilane columns.

Authors:  C Y Yang; F I Sepulveda
Journal:  J Chromatogr       Date:  1985-10-18
  10 in total

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