Literature DB >> 8419303

Analysis of the topology of a membrane protein by using a minimum number of alkaline phosphatase fusions.

D Boyd1, B Traxler, J Beckwith.   

Abstract

An approach to analyzing the topology of membrane proteins with alkaline phosphatase fusions is described. Precise fusions were constructed by using polymerase chain reaction at the C terminus of each hydrophilic region of the membrane protein. The disruption of topogenic signals is thereby minimized, and predictable anomalous results are avoided. The Escherichia coli MalG protein has been analyzed.

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Year:  1993        PMID： 8419303      PMCID： PMC196172          DOI： 10.1128/jb.175.2.553-556.1993

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  21 in total

Review 1.  Genetic basis of multidrug resistance of tumor cells.

Authors:  S E Kane; I Pastan; M M Gottesman
Journal:  J Bioenerg Biomembr       Date:  1990-08       Impact factor: 2.945

2.  The prediction of transmembrane protein sequences and their conformation: an evaluation.

Authors:  G D Fasman; W A Gilbert
Journal:  Trends Biochem Sci       Date:  1990-03       Impact factor: 13.807

3.  Genetic analysis of membrane protein topology by a sandwich gene fusion approach.

Authors:  M Ehrmann; D Boyd; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1990-10       Impact factor: 11.205

4.  lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion.

Authors:  J Calamia; C Manoil
Journal:  Proc Natl Acad Sci U S A       Date:  1990-07       Impact factor: 11.205

Review 5.  Bacterial periplasmic permeases belong to a family of transport proteins operating from Escherichia coli to human: Traffic ATPases.

Authors:  G F Ames; C S Mimura; V Shyamala
Journal:  FEMS Microbiol Rev       Date:  1990-08       Impact factor: 16.408

6.  Use of phoA fusions to study the topology of the Escherichia coli inner membrane protein leader peptidase.

Authors:  J L San Millan; D Boyd; R Dalbey; W Wickner; J Beckwith
Journal:  J Bacteriol       Date:  1989-10       Impact factor: 3.490

7.  A critical evaluation of the hydropathy profile of membrane proteins.

Authors:  M Degli Esposti; M Crimi; G Venturoli
Journal:  Eur J Biochem       Date:  1990-05-31

8.  Determinants of membrane protein topology.

Authors:  D Boyd; C Manoil; J Beckwith
Journal:  Proc Natl Acad Sci U S A       Date:  1987-12       Impact factor: 11.205

Review 9.  Binding protein-dependent transport systems.

Authors:  C F Higgins; S C Hyde; M M Mimmack; U Gileadi; D R Gill; M P Gallagher
Journal:  J Bioenerg Biomembr       Date:  1990-08       Impact factor: 2.945

10.  Analysis of the membrane organization of an Escherichia coli protein translocator, HlyB, a member of a large family of prokaryote and eukaryote surface transport proteins.

Authors:  R C Wang; S J Seror; M Blight; J M Pratt; J K Broome-Smith; I B Holland
Journal:  J Mol Biol       Date:  1991-02-05       Impact factor: 5.469
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  28 in total

1.  Mutational analysis and membrane topology of ComP, a quorum-sensing histidine kinase of Bacillus subtilis controlling competence development.

Authors:  F Piazza; P Tortosa; D Dubnau
Journal:  J Bacteriol       Date:  1999-08       Impact factor: 3.490

2.  Divergent evolution of membrane protein topology: the Escherichia coli RnfA and RnfE homologues.

Authors:  A Sääf; M Johansson; E Wallin; G von Heijne
Journal:  Proc Natl Acad Sci U S A       Date:  1999-07-20       Impact factor: 11.205

Review 3.  Membrane topology and insertion of membrane proteins: search for topogenic signals.

Authors:  M van Geest; J S Lolkema
Journal:  Microbiol Mol Biol Rev       Date:  2000-03       Impact factor: 11.056

4.  Membrane topology of the multidrug transporter MdfA: complementary gene fusion studies reveal a nonessential C-terminal domain.

Authors:  Julia Adler; Eitan Bibi
Journal:  J Bacteriol       Date:  2002-06       Impact factor: 3.490

5.  The secretory carrier membrane protein family: structure and membrane topology.

Authors:  C Hubbard; D Singleton; M Rauch; S Jayasinghe; D Cafiso; D Castle
Journal:  Mol Biol Cell       Date:  2000-09       Impact factor: 4.138

6.  Membrane topology of PssT, the transmembrane protein component of the type I exopolysaccharide transport system in Rhizobium leguminosarum bv. trifolii strain TA1.

Authors:  Andrzej Mazur; Jarosław E Król; Małgorzata Marczak; Anna Skorupska
Journal:  J Bacteriol       Date:  2003-04       Impact factor: 3.490

7.  Domain organization of the MscS mechanosensitive channel of Escherichia coli.

Authors:  Samantha Miller; Wendy Bartlett; Subramanian Chandrasekaran; Sally Simpson; Michelle Edwards; Ian R Booth
Journal:  EMBO J       Date:  2003-01-02       Impact factor: 11.598

Review 8.  Tinkering with transporters: periplasmic binding protein-dependent maltose transport in E. coli.

Authors:  H A Shuman; C H Panagiotidis
Journal:  J Bioenerg Biomembr       Date:  1993-12       Impact factor: 2.945

9.  Topological characterization of the essential Escherichia coli cell division protein FtsN.

Authors:  K Dai; Y Xu; J Lutkenhaus
Journal:  J Bacteriol       Date:  1996-03       Impact factor: 3.490

10.  Characterization of transmembrane domains 6, 7, and 8 of MalF by mutational analysis.

Authors:  R Ehrle; C Pick; R Ulrich; E Hofmann; M Ehrmann
Journal:  J Bacteriol       Date:  1996-04       Impact factor: 3.490
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