| Literature DB >> 8407809 |
M M Vickerman1, D G Heath, D B Clewell.
Abstract
Degenerate oligonucleotide primers were used in a polymerase chain reaction (PCR) to amplify a region of the recA sequence of Streptococcus gordonii Challis. The resulting PCR fragment was cloned into the suicide vector pAM6199 and introduced into strain Challis, giving rise to recombination-deficient strains in which the recA gene was specifically inactivated.Entities:
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Year: 1993 PMID: 8407809 PMCID: PMC206735 DOI: 10.1128/jb.175.19.6354-6357.1993
Source DB: PubMed Journal: J Bacteriol ISSN: 0021-9193 Impact factor: 3.490