Literature DB >> 8405153

Characterization of human faecal flora by means of an improved fluoro-morphometrical method.

G J Jansen1, M H Wilkinson, B Deddens, D van der Waaij.   

Abstract

The use of polyclonal antibodies in differentiating between samples of faecal microflora derived from ten healthy volunteers was assessed. The distribution of FITC-labelled antibodies (of the IgA, IgG and IgM isotype) over 144 morphologically distinct subsets of faecal bacteria ('morphotypes') was quantified by means of digital morphometry and quantitative immunofluorescence. Furthermore, a new dataprocessing algorithm was developed which makes objective quantitation of the antibody distributions over the faecal morphotypes possible. The results of this study imply that the antibody binding capacity of faecal morphotypes is a unique characteristic of faecal microflora.

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Year:  1993        PMID： 8405153      PMCID： PMC2271394          DOI： 10.1017/s0950268800056971

Source DB:  PubMed          Journal:  Epidemiol Infect        ISSN： 0950-2688            Impact factor:   2.451


  10 in total

1.  Circulating antibodies against faecal bacteria assessed by immunomorphometry: combining quantitative immunofluorescence and image analysis.

Authors:  H Z Apperloo-Renkema; M H Wilkinson; D van der Waaij
Journal:  Epidemiol Infect       Date:  1992-12       Impact factor: 2.451

2.  Effects of ceftriaxone on faecal flora: analysis by micromorphometry.

Authors:  B C Meijer; G J Kootstra; D G Geertsma; M H Wilkinson
Journal:  Epidemiol Infect       Date:  1991-06       Impact factor: 2.451

3.  The effect of ceftriaxone on the anaerobic bacterial flora and the bacterial enzymatic activity in the intestinal tract.

Authors:  G W Welling; G J Meijer-Severs; G Helmus; E van Santen; R H Tonk; H G de Vries-Hospers; D van der Waaij
Journal:  Infection       Date:  1991 Sep-Oct       Impact factor: 3.553

4.  Morphometrical parameters of gut microflora in human volunteers.

Authors:  B C Meijer; G J Kootstra; M H Wilkinson
Journal:  Epidemiol Infect       Date:  1991-10       Impact factor: 2.451

5.  Objective quantitation of serum antibody titres against Enterobacteriaceae using indirect immunofluorescence, read by videocamera and image processing system.

Authors:  H Z Apperloo-Renkema; M H Wilkinson; D G Oenema; D van der Waaij
Journal:  Med Microbiol Immunol       Date:  1991       Impact factor: 3.402

6.  Colonization resistance of the digestive tract in conventional and antibiotic-treated mice.

Authors:  D van der Waaij; J M Berghuis-de Vries
Journal:  J Hyg (Lond)       Date:  1971-09

7.  The effect of new broad-spectrum antibiotics on faecal flora of cancer patients.

Authors:  G J Meijer-Severs; J H Joshi
Journal:  J Antimicrob Chemother       Date:  1989-10       Impact factor: 5.790

8.  Selective gut decontamination reduces nosocomial infections and length of stay but not mortality or organ failure in surgical intensive care unit patients.

Authors:  F B Cerra; M A Maddaus; D L Dunn; C L Wells; N N Konstantinides; S L Lehmann; H J Mann
Journal:  Arch Surg       Date:  1992-02

Review 9.  Are intestinal bacteria involved in the etiology of rheumatoid arthritis? Review article.

Authors:  M P Hazenberg; I S Klasen; J Kool; J G Ruseler-van Embden; A J Severijnen
Journal:  APMIS       Date:  1992-01       Impact factor: 3.205

10.  Effect of broad-spectrum parenteral antibiotics on "colonization resistance" of intestinal microflora of humans.

Authors:  M Barza; M Giuliano; N V Jacobus; S L Gorbach
Journal:  Antimicrob Agents Chemother       Date:  1987-05       Impact factor: 5.191
  10 in total
  3 in total

1.  Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples.

Authors:  P S Langendijk; F Schut; G J Jansen; G C Raangs; G R Kamphuis; M H Wilkinson; G W Welling
Journal:  Appl Environ Microbiol       Date:  1995-08       Impact factor: 4.792

2.  Monoclonal immunoglobulin A derived from peritoneal B cells is encoded by both germ line and somatically mutated VH genes and is reactive with commensal bacteria.

Authors:  N A Bos; J C Bun; S H Popma; E R Cebra; G J Deenen; M J van der Cammen; F G Kroese; J J Cebra
Journal:  Infect Immun       Date:  1996-02       Impact factor: 3.441

Review 3.  Specific antibody activity, glycan heterogeneity and polyreactivity contribute to the protective activity of S-IgA at mucosal surfaces.

Authors:  Jiri Mestecky; Michael W Russell
Journal:  Immunol Lett       Date:  2009-04-05       Impact factor: 3.685
  3 in total

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