Literature DB >> 8395226

Application of enzyme-linked immunosorbent assay for the seroepizootiological survey of antibodies against porcine cytomegalovirus.

T Tajima1, T Hironao, T Kajikawa, H Kawamura.   

Abstract

Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against porcine cytomegalovirus (PCMV) was developed using the OF-1 strain of PCMV as an antigen. Results of the ELISA were compared to those of indirect fluorescent antibody (IFA) and serum neutralization (SN) tests. ELISA and IFA test were found to be extremely sensitive more than SN test. All of 11 tested sera were highly reactive in both ELISA and IFA test, but 6 of them were antibody-negative in the SN test. The retrospective survey of 436 fattening pig sera collected in Japan in 1981 showed that 433 (99.4%) of them were highly antibody-positive in ELISA.

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Year:  1993        PMID： 8395226     DOI： 10.1292/jvms.55.421

Source DB:  PubMed          Journal:  J Vet Med Sci        ISSN： 0916-7250            Impact factor:   1.267


  6 in total

1.  Quantitation of porcine cytomegalovirus in pig tissues by PCR.

Authors:  J F Fryer; P D Griffiths; J A Fishman; V C Emery; D A Clark
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

2.  PCR assay for detecting porcine cytomegalovirus.

Authors:  A L Hamel; L Lin; C Sachvie; E Grudeski; G P Nayar
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

3.  Identification and sequence analysis of the glycoprotein B gene of porcine cytomegalovirus.

Authors:  F Widen; M Goltz; N Wittenbrink; B Ehlers; M Banks; S Belak
Journal:  Virus Genes       Date:  2001-12       Impact factor: 2.332

4.  Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.

Authors:  Jin-Long Yang; Su-Hui Zhang; Zuo-Hua Liu; Rui Yang; Yong Huang; Ming Wen
Journal:  Virol J       Date:  2012-12-29       Impact factor: 4.099

5.  Porcine cytomegalovirus infection is not associated to the occurrence of post-weaning multisystemic wasting syndrome.

Authors:  Samuel Paulo Cibulski; Gabriela Pasqualim; Thais Fumaco Teixeira; Ana Paula Muterle Varela; Diogenes Dezen; Carine Lidiane Holz; Ana Cláudia Franco; Paulo Michel Roehe
Journal:  Vet Med Sci       Date:  2015-07-07

6.  Development of a TaqMan Based Real-Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen.

Authors:  Rujing Chen; Qiuyong Chen; Xuemin Wu; Yongliang Che; Chenyan Wang; Longbai Wang; Shan Yan; Lunjiang Zhou
Journal:  Biomed Res Int       Date:  2020-01-10       Impact factor: 3.411
  6 in total

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