Literature DB >> 10523598

PCR assay for detecting porcine cytomegalovirus.

A L Hamel1, L Lin, C Sachvie, E Grudeski, G P Nayar.   

Abstract

This is the first published report of a PCR assay for detecting porcine cytomegalovirus (PCMV), the causative agent of inclusion body rhinitis in pigs. The DNA to be tested was extracted directly from lungs and nasal scrapings of pigs with various clinical syndromes. Fifty-nine percent (74 of 126) of tested pigs with various clinical syndromes were found to be PCR positive for PCMV. It is hoped that veterinary diagnostic laboratories will benefit by using this PCR assay for routine testing and surveillance of PCMV in pigs.

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Year:  1999        PMID： 10523598      PMCID： PMC85757     

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Basic local alignment search tool.

Authors:  S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
Journal:  J Mol Biol       Date:  1990-10-05       Impact factor: 5.469

2.  Morphologic study of inclusions in tissues from pigs inoculated with cytomegalovirus.

Authors:  M Narita; H Kawamura; J Shirai; M Haritani
Journal:  Am J Vet Res       Date:  1987-09       Impact factor: 1.156

3.  Differentiation between porcine reproductive and respiratory syndrome virus isolates by restriction fragment length polymorphism of their ORFs 6 and 7 genes.

Authors:  C A Gagnon; S Dea
Journal:  Can J Vet Res       Date:  1998-04       Impact factor: 1.310

Review 4.  Sequence-based identification of microbial pathogens: a reconsideration of Koch's postulates.

Authors:  D N Fredricks; D A Relman
Journal:  Clin Microbiol Rev       Date:  1996-01       Impact factor: 26.132

5.  Detection and characterization of porcine circovirus associated with postweaning multisystemic wasting syndrome in pigs.

Authors:  G P Nayar; A Hamel; L Lin
Journal:  Can Vet J       Date:  1997-06       Impact factor: 1.008

6.  Evidence for circovirus in cattle with respiratory disease and from aborted bovine fetuses.

Authors:  G P Nayar; A L Hamel; L Lin; C Sachvie; E Grudeski; G Spearman
Journal:  Can Vet J       Date:  1999-04       Impact factor: 1.008

7.  Indirect fluorescence antibody studies of porcine cytomegalo virus infections in the Netherlands.

Authors:  P R Rondhuis; M F de Jong; J Schep
Journal:  Tijdschr Diergeneeskd       Date:  1980-04-15

8.  Application of enzyme-linked immunosorbent assay for the seroepizootiological survey of antibodies against porcine cytomegalovirus.

Authors:  T Tajima; T Hironao; T Kajikawa; H Kawamura
Journal:  J Vet Med Sci       Date:  1993-06       Impact factor: 1.267

9.  Rapid detection of bovine viral diarrhea virus by using RNA extracted directly from assorted specimens and a one-tube reverse transcription PCR assay.

Authors:  A L Hamel; M D Wasylyshen; G P Nayar
Journal:  J Clin Microbiol       Date:  1995-02       Impact factor: 5.948

10.  Enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to porcine cytomegalovirus.

Authors:  R Assaf; A M Bouillant; E Di Franco
Journal:  Can J Comp Med       Date:  1982-04
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  15 in total

1.  Quantitation of porcine cytomegalovirus in pig tissues by PCR.

Authors:  J F Fryer; P D Griffiths; J A Fishman; V C Emery; D A Clark
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

2.  Characterization of a new disease syndrome associated with porcine circovirus type 2 in previously vaccinated herds.

Authors:  Ada G Cino-Ozuna; Steven Henry; Richard Hesse; Jerome C Nietfeld; Jianfa Bai; H Morgan Scott; Raymond R R Rowland
Journal:  J Clin Microbiol       Date:  2011-02-23       Impact factor: 5.948

Review 3.  Xenotransplantation and the potential risk of xenogeneic transmission of porcine viruses.

Authors:  D Yoo; A Giulivi
Journal:  Can J Vet Res       Date:  2000-10       Impact factor: 1.310

4.  Monitoring for presence of potentially xenotic viruses in recipients of pig islet xenotransplantation.

Authors:  O Garkavenko; M C Croxson; M Irgang; A Karlas; J Denner; R B Elliott
Journal:  J Clin Microbiol       Date:  2004-11       Impact factor: 5.948

5.  Characterization of the DNA polymerase loci of porcine cytomegaloviruses from diverse geographic origins.

Authors:  M Goltz; F Widen; M Banks; S Belak; B Ehlers
Journal:  Virus Genes       Date:  2000-10       Impact factor: 2.332

6.  Reactivation of porcine cytomegalovirus through allogeneic stimulation.

Authors:  Maria Isabel M C Guedes; Jack M Risdahl; Barry Wiseman; Thomas W Molitor
Journal:  J Clin Microbiol       Date:  2004-04       Impact factor: 5.948

7.  Development and evaluation of a loop-mediated isothermal amplification assay for the rapid detection of porcine cytomegalovirus under field conditions.

Authors:  Jin-Long Yang; Su-Hui Zhang; Zuo-Hua Liu; Rui Yang; Yong Huang; Ming Wen
Journal:  Virol J       Date:  2012-12-29       Impact factor: 4.099

8.  Antibody Cross-Reactivity between Porcine Cytomegalovirus (PCMV) and Human Herpesvirus-6 (HHV-6).

Authors:  Uwe Fiebig; Angela Holzer; Daniel Ivanusic; Elena Plotzki; Hartmut Hengel; Frank Neipel; Joachim Denner
Journal:  Viruses       Date:  2017-10-28       Impact factor: 5.048

9.  Attempted experimental reproduction of porcine periweaning-failure-to-thrive syndrome using tissue homogenates.

Authors:  Yanyun Huang; John C S Harding
Journal:  PLoS One       Date:  2014-03-03       Impact factor: 3.240

10.  No evidence of viral transmission following long-term implantation of agarose encapsulated porcine islets in diabetic dogs.

Authors:  Lawrence S Gazda; Horatiu V Vinerean; Melissa A Laramore; Richard D Hall; Joseph W Carraway; Barry H Smith
Journal:  J Diabetes Res       Date:  2014-06-05       Impact factor: 4.011
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