Literature DB >> 8393567

A cellular factor stimulates the DNA-binding activity of MyoD and E47.

M J Thayer1, H Weintraub.   

Abstract

We show that mixing purified MyoD and E47 proteins results in heterodimers that fail to bind DNA, even though MyoD and E47 homodimers can bind DNA efficiently. Addition of cell extracts or a specific fraction from a cell extract enables the heterodimer to bind DNA, but components in this fraction fail to enter the DNA complex. The activity is sensitive to heat and protease and is not ATP-dependent. The activity functions on E47 and MyoD homodimers and can stimulate DNA binding of the basic-helix-loop-helix region of MyoD. The effectiveness of the activity, for MyoD homodimers, depends on the exact DNA sequence of the binding site. Our results suggest that specific factors in the cell might control the DNA-binding properties of helix-loop-helix proteins.

Entities:  

Mesh：

Substances：

Year:  1993        PMID： 8393567      PMCID： PMC46956          DOI： 10.1073/pnas.90.14.6483

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  19 in total

1.  Common denominators for sex.

Authors:  S M Parkhurst; D Ish-Horowicz
Journal:  Curr Biol       Date:  1992-12       Impact factor: 10.834

2.  The GCN4 basic region leucine zipper binds DNA as a dimer of uninterrupted alpha helices: crystal structure of the protein-DNA complex.

Authors:  T E Ellenberger; C J Brandl; K Struhl; S C Harrison
Journal:  Cell       Date:  1992-12-24       Impact factor: 41.582

3.  MyoD is a sequence-specific DNA binding protein requiring a region of myc homology to bind to the muscle creatine kinase enhancer.

Authors:  A B Lassar; J N Buskin; D Lockshon; R L Davis; S Apone; S D Hauschka; H Weintraub
Journal:  Cell       Date:  1989-09-08       Impact factor: 41.582

4.  Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence.

Authors:  C Murre; P S McCaw; H Vaessin; M Caudy; L Y Jan; Y N Jan; C V Cabrera; J N Buskin; S D Hauschka; A B Lassar
Journal:  Cell       Date:  1989-08-11       Impact factor: 41.582

5.  T7 lysozyme inhibits transcription by T7 RNA polymerase.

Authors:  B A Moffatt; F W Studier
Journal:  Cell       Date:  1987-04-24       Impact factor: 41.582

6.  The Drosophila extramacrochaetae locus, an antagonist of proneural genes that, like these genes, encodes a helix-loop-helix protein.

Authors:  J Garrell; J Modolell
Journal:  Cell       Date:  1990-04-06       Impact factor: 41.582

7.  Evidence that sisterless-a and sisterless-b are two of several discrete "numerator elements" of the X/A sex determination signal in Drosophila that switch Sxl between two alternative stable expression states.

Authors:  T W Cline
Journal:  Genetics       Date:  1988-08       Impact factor: 4.562

8.  Identification of a myocyte nuclear factor that binds to the muscle-specific enhancer of the mouse muscle creatine kinase gene.

Authors:  J N Buskin; S D Hauschka
Journal:  Mol Cell Biol       Date:  1989-06       Impact factor: 4.272

9.  A novel human muscle factor related to but distinct from MyoD1 induces myogenic conversion in 10T1/2 fibroblasts.

Authors:  T Braun; G Buschhausen-Denker; E Bober; E Tannich; H H Arnold
Journal:  EMBO J       Date:  1989-03       Impact factor: 11.598

10.  Myf-6, a new member of the human gene family of myogenic determination factors: evidence for a gene cluster on chromosome 12.

Authors:  T Braun; E Bober; B Winter; N Rosenthal; H H Arnold
Journal:  EMBO J       Date:  1990-03       Impact factor: 11.598
View more
  16 in total

1.  Establishment of distinct MyoD, E2A, and twist DNA binding specificities by different basic region-DNA conformations.

Authors:  T Kophengnavong; J E Michnowicz; T K Blackwell
Journal:  Mol Cell Biol       Date:  2000-01       Impact factor: 4.272

2.  Interaction between acetylated MyoD and the bromodomain of CBP and/or p300.

Authors:  A Polesskaya; I Naguibneva; A Duquet; E Bengal; P Robin; A Harel-Bellan
Journal:  Mol Cell Biol       Date:  2001-08       Impact factor: 4.272

3.  Degradation of myogenic transcription factor MyoD by the ubiquitin pathway in vivo and in vitro: regulation by specific DNA binding.

Authors:  O Abu Hatoum; S Gross-Mesilaty; K Breitschopf; A Hoffman; H Gonen; A Ciechanover; E Bengal
Journal:  Mol Cell Biol       Date:  1998-10       Impact factor: 4.272

4.  MyoD forms micelles which can dissociate to form heterodimers with E47: implications of micellization on function.

Authors:  T M Laue; M A Starovasnik; H Weintraub; X H Sun; L Snider; R E Klevit
Journal:  Proc Natl Acad Sci U S A       Date:  1995-12-05       Impact factor: 11.205

5.  A cellular factor stimulates ligand-dependent release of hsp90 from the basic helix-loop-helix dioxin receptor.

Authors:  J McGuire; M L Whitelaw; I Pongratz; J A Gustafsson; L Poellinger
Journal:  Mol Cell Biol       Date:  1994-04       Impact factor: 4.272

6.  Cellular aggregation enhances MyoD-directed skeletal myogenesis in embryonal carcinoma cells.

Authors:  I S Skerjanc; R S Slack; M W McBurney
Journal:  Mol Cell Biol       Date:  1994-12       Impact factor: 4.272

7.  The presence of transcription factors in chicken albumin, yolk and blastoderm.

Authors:  P A Knepper; C S Mayanil; E Hayes; W Goossens; R W Byrne; D G McLone
Journal:  In Vitro Cell Dev Biol Anim       Date:  1999-06       Impact factor: 2.416

8.  Cell transformation by c-fos requires an extended period of expression and is independent of the cell cycle.

Authors:  G G Miao; T Curran
Journal:  Mol Cell Biol       Date:  1994-06       Impact factor: 4.272

9.  A novel site for ubiquitination: the N-terminal residue, and not internal lysines of MyoD, is essential for conjugation and degradation of the protein.

Authors:  K Breitschopf; E Bengal; T Ziv; A Admon; A Ciechanover
Journal:  EMBO J       Date:  1998-10-15       Impact factor: 11.598

10.  Affinity enrichment and functional characterization of TRAX1, a novel transcription activator and X1-sequence-binding protein of HLA-DRA.

Authors:  Y Itoh-Lindstrom; B M Peterlin; J P Ting
Journal:  Mol Cell Biol       Date:  1995-01       Impact factor: 4.272
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.