Literature DB >> 8390581

Crystallization of alpha-galactosidase from Trichoderma reesei.

A M Golubev1, K N Neustroev.   

Abstract

An extracellular alpha-galactosidase was isolated from fungus Trichoderma reesei. The purified enzyme had a molecular weight of 54,000 Da and an isoelectric point of 5.25. Crystals of alpha-galactosidase were obtained from a polyethylene glycol 4000 solution by the hanging-drop method. Seeding was used for enlargement of the crystal size. The crystals belong to the orthorhombic space group P2(1)2(1)2 with cell dimensions a = 122.2 A, b = 81.0 A c = 49.7 A and diffract beyond 3.0 A resolution.

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Year:  1993        PMID： 8390581     DOI： 10.1006/jmbi.1993.1340

Source DB:  PubMed          Journal:  J Mol Biol        ISSN： 0022-2836            Impact factor:   5.469


  4 in total

1.  The carbohydrate moiety of alpha-galactosidase from Trichoderma reesei.

Authors:  A N Savel'ev; E V Eneyskaya; L S Isaeva-Ivanova; K A Shabalin; A M Golubev; K N Neustroev
Journal:  Glycoconj J       Date:  1997-12       Impact factor: 2.916

2.  Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360.

Authors:  O Fridjonsson; H Watzlawick; A Gehweiler; T Rohrhirsch; R Mattes
Journal:  Appl Environ Microbiol       Date:  1999-09       Impact factor: 4.792

3.  Role of methionine in the active site of alpha-galactosidase from Trichoderma reesei.

Authors:  A M Kachurin; A M Golubev; M M Geisow; O S Veselkina; L S Isaeva-Ivanova; K N Neustroev
Journal:  Biochem J       Date:  1995-06-15       Impact factor: 3.857

4.  Cloning and high-level expression of alpha-galactosidase cDNA from Penicillium purpurogenum.

Authors:  H Shibuya; H Nagasaki; S Kaneko; S Yoshida; G G Park; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792
  4 in total

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