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Literature DB >> 8390475

Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification.

Abstract

Genomic RNA fingerprints of infectious bronchitis virus (IBV) strains M41 and Conn46 were prepared to identify T1 RNase-resistant oligonucleotides 'unique' to each of the two IBV strains. Such oligonucleotides were subsequently eluted from the gels and their nucleotide sequences determined. When oligonucleotide probes of those sequences were synthesized and used in a dot-blot hybridization assay, the probes lacked IBV strain-specificity and reacted with the RNAs of homologous as well as heterologous IBV strains. Based on these results, the methods used in this study need to be applied to a large number of oligonucleotide probes, to find one or a few that might be suitable as IBV strain- or serotype-specific oligonucleotide probes.

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Year: 1993 PMID： 8390475 PMCID： PMC7119523 DOI： 10.1016/0166-0934(93)90040-x

Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014

19 in total

1. Confirmation of the presence of avian infectious bronchitis virus (IBV) using cloned DNA complementary to the 3' terminus of the IBV genome.

Authors: T D Brown; D Cavanagh; M E Boursnell
Journal: Avian Pathol Date: 1984-01 Impact factor: 3.378

2. Dot blot detection of point mutations with adjacently hybridising synthetic oligonucleotide probes.

Authors: A M Alves; F J Carr
Journal: Nucleic Acids Res Date: 1988-09-12 Impact factor: 16.971

3. A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism.

Authors: Z Lin; A Kato; Y Kudou; S Ueda
Journal: Arch Virol Date: 1991 Impact factor: 2.574

1. Rapid differentiation of avian infectious bronchitis virus isolates by sample to residual ratio quantitation using real-time reverse transcriptase-polymerase chain reaction.

Authors: S A Callison; D A Hilt; M W Jackwood
Journal: J Virol Methods Date: 2005-01-06 Impact factor: 2.014

1 in total

Oligonucleotide probes in infectious bronchitis virus diagnosis and strain identification.

1. Confirmation of the presence of avian infectious bronchitis virus (IBV) using cloned DNA complementary to the 3' terminus of the IBV genome.

2. Dot blot detection of point mutations with adjacently hybridising synthetic oligonucleotide probes.

3. A new typing method for the avian infectious bronchitis virus using polymerase chain reaction and restriction enzyme fragment length polymorphism.

4. Molecular epidemiology of infectious bronchitis virus in The Netherlands.

5. Serological and molecular characterization of three enteric isolates of infectious bronchitis virus of chickens.

6. Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins.

7. An oligonucleotide probe for the detection of hepatitis B virus DNA in serum.

8. Chrysanthemum stunt viroid: primary sequence and secondary structure.

9. Persistent measles virus genome in autoimmune chronic active hepatitis.

10. Rapid detection and identification of avian infectious bronchitis virus.

1. Rapid differentiation of avian infectious bronchitis virus isolates by sample to residual ratio quantitation using real-time reverse transcriptase-polymerase chain reaction.