| Literature DB >> 8388400 |
S Vilcek1.
Abstract
The amplification of the 468 bp fragment of the BHV-1 genome by PCR is described. The 22 bp oligomers from the BHV-1 gI gene were used as primers. For successful amplification the thermal denaturation (100 degrees C/8 min, ice) of the DNA sample was carried out prior to the cycling (95 degrees C for 1 min, 56 degrees C for 1 min, 73 degrees C for 1 min). The presence of glycerol markedly enhances the PCR.Entities:
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Year: 1993 PMID： 8388400 DOI： 10.1016/0166-0934(93)90132-b
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014