| Literature DB >> 14582748 |
L I Gomes1, M A Rocha, J G Souza, E A Costa, E F Barbosa-Stancioli.
Abstract
Bovine herpesvirus type 5 (BoHV-5), which is potentially neuropathogenic, was detected in clinical samples of bovine semen, both directly and after isolation in cell culture, using a nested PCR system for amplifying the US4 gene. Nucleotide sequences generated from the amplicons were analysed and deposited at GenBank (NCBI, Bethesda, MD, USA) under the accession numbers AF298174 and AF330157. Alignment of these sequences and previously deposited sequences of BoHV-1 and BoHV-5 showed 82% and 98% similarity, respectively. The bulls, which were maintained at an artificial insemination centre, had presented no clinical signs, indicating that bovine semen should be screened for BoHV-5 to prevent transmission of the virus.Entities:
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Year: 2003 PMID： 14582748 DOI： 10.1023/a:1025745825610
Source DB: PubMed Journal: Vet Res Commun ISSN： 0165-7380 Impact factor: 2.459