Literature DB >> 8384623

Direct injection assay of angiotensin-converting enzyme by high-performance liquid chromatography using a shielded hydrophobic phase column.

M T Doig1, J W Smiley.   

Abstract

A rapid and sensitive method for the determination of angiotensin-converting enzyme activity in serum and tissue extracts is described. The procedure is based on the high-performance liquid chromatographic separation of the synthetic substrate hippuryl-L-histidyl-L-leucine from the hydrolysis product hippuric acid. The separation is accomplished by direct injection of biological assay mixtures onto a shielded hydrophobic phase column with isocratic elution.

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Year:  1993        PMID： 8384623     DOI： 10.1016/0378-4347(93)80208-l

Source DB:  PubMed          Journal:  J Chromatogr


  4 in total

1.  A General, Label-Free and Homogeneous Electrochemical Strategy for Probing of Protease Activity and Screening of Inhibitor.

Authors:  Yunxiao Feng; Gang Liu; Fan Zhang; Jianwen Liu; Ming La; Ning Xia
Journal:  Micromachines (Basel)       Date:  2022-05-21       Impact factor: 3.523

2.  A Convenient RP-HPLC Method for Assay Bioactivities of Angiotensin I-Converting Enzyme Inhibitory Peptides.

Authors:  Wei Wang; Nan Wang; Yu Zhang; Zheng Cai; Qihe Chen; Guoqing He
Journal:  ISRN Biotechnol       Date:  2012-10-21

3.  Bioactivity-Guided Fractionation of Pine Needle Reveals Catechin as an Anti-hypertension Agent via Inhibiting Angiotensin-Converting Enzyme.

Authors:  Jian He
Journal:  Sci Rep       Date:  2017-08-21       Impact factor: 4.379

Review 4.  Review of Angiotensin-converting Enzyme Inhibitory Assay: Rapid Method in Drug Discovery of Herbal Plants.

Authors:  Islamudin Ahmad; Arry Yanuar; Kamarza Mulia; Abdul Mun'im
Journal:  Pharmacogn Rev       Date:  2017 Jan-Jun
  4 in total

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