Literature DB >> 8382477

Organization of the phosphoinositide cycle. Assessment of inositol transferase activity in purified plasma membranes.

O M Santiago1, L I Rosenberg, M E Monaco.   

Abstract

Experiments were carried out to determine whether or not CDP-diacylglycerol:myo-inositol 3-phosphatidyltransferase (IT) activity (EC 2.7.8.11) could be detected in purified plasma-membrane fractions from WRK-1 rat mammary tumour cells. These cells have previously been shown to have a very active phosphoinositide cycle. Sucrose-density-gradient-purified plasma membranes contained no IT activity that could not be accounted for by endoplasmic-reticulum contamination. However, we also determined that the relative amount of IT activity in endoplasmic reticulum and plasma-membrane fractions could be altered by changing the concentration of detergent in the assay system.

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Year:  1993        PMID： 8382477      PMCID： PMC1132399          DOI： 10.1042/bj2900179

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  24 in total

1.  Solubilization of the enzyme catalyzing CDP-diglyceride-independent incorporation of myo-inositol into phosphatidyl inositol and its comparison to CDP-diglyceride:inositol transferase.

Authors:  T Takenawa; M Saito; Y Nagai; K Egawa
Journal:  Arch Biochem Biophys       Date:  1977-07       Impact factor: 4.013

2.  Unsaturated fatty acid requirements for growth and survival of a rat mammary tumor cell line.

Authors:  W R Kidwell; M E Monaco; M S Wicha; G S Smith
Journal:  Cancer Res       Date:  1978-11       Impact factor: 12.701

3.  Distribution of phospholipid biosynthetic enzymes among cell components of rat liver.

Authors:  C L Jelsema; D J Morré
Journal:  J Biol Chem       Date:  1978-11-10       Impact factor: 5.157

4.  Protein measurement with the Folin phenol reagent.

Authors:  O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal:  J Biol Chem       Date:  1951-11       Impact factor: 5.157

5.  Evidence for the biosynthetic difference between isolated mitochondria and microsomes from guinea-pig and rat liver regarding lysophosphatidic acid, phosphatidic acid, CDP-diglyceride, phosphatidylglycerol, and cardiolipin.

Authors:  J B Davidson; N Z Stanacev
Journal:  Can J Biochem       Date:  1974-10

6.  Biosynthesis of lipids in Golgi complex and other subcellular fractions from rat liver.

Authors:  L M van Golde; J Raben; J J Batenburg; B Fleischer; F Zambrano; S Fleischer
Journal:  Biochim Biophys Acta       Date:  1974-08-22

7.  The phosphatidylinositol cycle in WRK-1 cells. Evidence for a separate, hormone-sensitive phosphatidylinositol pool.

Authors:  M E Monaco
Journal:  J Biol Chem       Date:  1982-03-10       Impact factor: 5.157

8.  Subcellular incorporation of 32P into phosphoinositides and other phospholipids in isolated hepatocytes.

Authors:  M A Seyfred; W W Wells
Journal:  J Biol Chem       Date:  1984-06-25       Impact factor: 5.157

9.  Subcellular site and mechanism of vasopressin-stimulated hydrolysis of phosphoinositides in rat hepatocytes.

Authors:  M A Seyfred; W W Wells
Journal:  J Biol Chem       Date:  1984-06-25       Impact factor: 5.157

10.  Enzymatic synthesis of cytidine diphosphate diglyceride.

Authors:  J R Carter; E P Kennedy
Journal:  J Lipid Res       Date:  1966-09       Impact factor: 5.922
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  4 in total

Review 1.  The interface between phosphatidylinositol transfer protein function and phosphoinositide signaling in higher eukaryotes.

Authors:  Aby Grabon; Vytas A Bankaitis; Mark I McDermott
Journal:  J Lipid Res       Date:  2018-11-30       Impact factor: 5.922

2.  Resynthesis of phosphatidylinositol in permeabilized neutrophils following phospholipase Cbeta activation: transport of the intermediate, phosphatidic acid, from the plasma membrane to the endoplasmic reticulum for phosphatidylinositol resynthesis is not dependent on soluble lipid carriers or vesicular transport.

Authors:  J Whatmore; C Wiedemann; P Somerharju; P Swigart; S Cockcroft
Journal:  Biochem J       Date:  1999-07-15       Impact factor: 3.857

3.  Evidence for a model of integrated inositol phospholipid pools implies an essential role for lipid transport in the maintenance of receptor-mediated phospholipase C activity in 1321N1 cells.

Authors:  I H Batty; R A Currie; C P Downes
Journal:  Biochem J       Date:  1998-03-15       Impact factor: 3.857

4.  Identification of rat liver phosphatidylinositol synthase as a 21 kDa protein.

Authors:  M E Monaco; M Feldman; D L Kleinberg
Journal:  Biochem J       Date:  1994-11-15       Impact factor: 3.857
  4 in total

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