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Literature DB >> 83813

Large-scale production of mouse mammary tumor virus in the absence of endogenous murine leukemia virus.

C V Benton, J S Harshman, O R Robinson, G P Shibley.

Abstract

A system for the large-scale production and purification of mouse mammary tumor virus in the absence of detectable endogenous murine leukemia virus is described. By utilizing the Mm5mt/c1 cell line established from an adenocarcinoma of a C3H mouse, the continuous production of over 25,000 liters of mouse mammary tumor virus-containing tissue culture fluids has been achieved. By the strict adherence to well-defined tissue culture conditions, mammary tumor virus production was accomplished without the expression of murine leukemia virus. Various biochemical and immunological systems were established for the rapid and precise detection of the endogenous leukemia virus, the expression of which could be enhanced under conditions of culture stress.

Entities: Disease Species

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Year: 1979 PMID： 83813 PMCID： PMC243415 DOI： 10.1128/aem.37.1.148-158.1979

Source DB: PubMed Journal: Appl Environ Microbiol ISSN： 0099-2240 Impact factor: 4.792

28 in total

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

Authors: F C GREENWOOD; W M HUNTER; J S GLOVER
Journal: Biochem J Date: 1963-10 Impact factor: 3.857

2. Polypeptides of the mouse mammary tumor virus. II. Identification of two major glycoproteins with the viral structure.

Authors: N H Sarkar; N E Taraschi; A A Pomenti; A S Dion
Journal: Virology Date: 1976-02 Impact factor: 3.616

3. Protein measurement with the Folin phenol reagent.

Authors: O H LOWRY; N J ROSEBROUGH; A L FARR; R J RANDALL
Journal: J Biol Chem Date: 1951-11 Impact factor: 5.157

4. Enhanced production of mouse mammary tumor virus in dexamethasone-treated, 5-iododeoxyuridine-stimulated mammary tumor cell cultures.

Authors: D L Fine; J K Plowman; S P Kelley; L O Arthur; E A Hillman
Journal: J Natl Cancer Inst Date: 1974-06 Impact factor: 13.506

Large-scale production of mouse mammary tumor virus in the absence of endogenous murine leukemia virus.

1. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.

2. Polypeptides of the mouse mammary tumor virus. II. Identification of two major glycoproteins with the viral structure.

3. Protein measurement with the Folin phenol reagent.

4. Enhanced production of mouse mammary tumor virus in dexamethasone-treated, 5-iododeoxyuridine-stimulated mammary tumor cell cultures.

5. Spontaneous production of a C-type RNA virus in rat tissue culture lines.

6. Conditions for optimal MTV synthesis in vitro and the effect of steroid hormones on virus production.

7. In vitro production of mouse mammary tumor virus in a mouse mammary tumor ascites line.

8. Spontaneous release of type C viruses from clonal lines of spontaneously transformed Blab-3T3 cells.

9. Rapid semiquantitative method for screening large numbers of virus samples by negative staining electron microscopy.

10. In vitro system for production of mouse mammary tumor virus.

1. New method for large-scale growth; and concentration of the Epstein-Barr viruses.