Literature DB >> 8367302

RAD50 protein of S.cerevisiae exhibits ATP-dependent DNA binding.

W E Raymond1, N Kleckner.   

Abstract

RAD50 function of Saccharomyces cerevisiae is required during vegetative growth for recombinational repair of DNA double strand breaks, and during meiosis for initiation of meiotic recombination and formation of synaptonemal complex. RAD50 encodes a 153 kDa polypeptide which includes an amino-terminal ATP binding domain essential for function and two long heptad repeat regions. We show below that RAD50 protein purified from yeast exhibits ATP-dependent binding to double stranded DNA. Physical properties of the purified protein are also described. Models for RAD50 function in vivo are discussed.

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Year:  1993        PMID： 8367302      PMCID： PMC309907          DOI： 10.1093/nar/21.16.3851

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  32 in total

1.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

2.  Three forms of the 5.8-S ribosomal RNA species in Saccharomyces cerevisiae.

Authors:  G M Rubin
Journal:  Eur J Biochem       Date:  1974-01-03

3.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

4.  Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.

Authors:  L M Siegel; K J Monty
Journal:  Biochim Biophys Acta       Date:  1966-02-07

5.  Conformational states of smooth muscle myosin. Effects of light chain phosphorylation and ionic strength.

Authors:  K M Trybus; S Lowey
Journal:  J Biol Chem       Date:  1984-07-10       Impact factor: 5.157

6.  Recombinationless meiosis in Saccharomyces cerevisiae.

Authors:  R E Malone; R E Esposito
Journal:  Mol Cell Biol       Date:  1981-10       Impact factor: 4.272

7.  A bent monomeric conformation of myosin from smooth muscle.

Authors:  K M Trybus; T W Huiatt; S Lowey
Journal:  Proc Natl Acad Sci U S A       Date:  1982-10       Impact factor: 11.205

8.  Expression of the Saccharomyces cerevisiae RAD50 gene during meiosis: steady-state transcript levels rise and fall while steady-state protein levels remain constant.

Authors:  W E Raymond; N Kleckner
Journal:  Mol Gen Genet       Date:  1993-04

9.  XRS2, a DNA repair gene of Saccharomyces cerevisiae, is needed for meiotic recombination.

Authors:  E L Ivanov; V G Korolev; F Fabre
Journal:  Genetics       Date:  1992-11       Impact factor: 4.562

10.  Identification of new genes required for meiotic recombination in Saccharomyces cerevisiae.

Authors:  M Ajimura; S H Leem; H Ogawa
Journal:  Genetics       Date:  1993-01       Impact factor: 4.562
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  42 in total

1.  Nbs1 potentiates ATP-driven DNA unwinding and endonuclease cleavage by the Mre11/Rad50 complex.

Authors:  T T Paull; M Gellert
Journal:  Genes Dev       Date:  1999-05-15       Impact factor: 11.361

2.  DNA end-binding specificity of human Rad50/Mre11 is influenced by ATP.

Authors:  Martijn de Jager; Claire Wyman; Dik C van Gent; Roland Kanaar
Journal:  Nucleic Acids Res       Date:  2002-10-15       Impact factor: 16.971

3.  Mre11 and Rad50 from Pyrococcus furiosus: cloning and biochemical characterization reveal an evolutionarily conserved multiprotein machine.

Authors:  K P Hopfner; A Karcher; D Shin; C Fairley; J A Tainer; J P Carney
Journal:  J Bacteriol       Date:  2000-11       Impact factor: 3.490

4.  Effect of rad50 mutation on illegitimate recombination in Saccharomyces cerevisiae.

Authors:  Cecilia Y Chan; Jie Zhu; Robert H Schiestl
Journal:  Mol Genet Genomics       Date:  2011-04-22       Impact factor: 3.291

5.  DDB2 complex-mediated ubiquitylation around DNA damage is oppositely regulated by XPC and Ku and contributes to the recruitment of XPA.

Authors:  Arato Takedachi; Masafumi Saijo; Kiyoji Tanaka
Journal:  Mol Cell Biol       Date:  2010-04-05       Impact factor: 4.272

6.  ATP-dependent DNA binding, unwinding, and resection by the Mre11/Rad50 complex.

Authors:  Yaqi Liu; Sihyun Sung; Youngran Kim; Fuyang Li; Gwanghyun Gwon; Aera Jo; Ae-Kyoung Kim; Taeyoon Kim; Ok-Kyu Song; Sang Eun Lee; Yunje Cho
Journal:  EMBO J       Date:  2015-12-30       Impact factor: 11.598

Review 7.  MRN and the race to the break.

Authors:  Agnieszka Rupnik; Noel F Lowndes; Muriel Grenon
Journal:  Chromosoma       Date:  2009-10-28       Impact factor: 4.316

8.  Relationship between nuclease-hypersensitive sites and meiotic recombination hot spot activity at the HIS4 locus of Saccharomyces cerevisiae.

Authors:  Q Q Fan; T D Petes
Journal:  Mol Cell Biol       Date:  1996-05       Impact factor: 4.272

9.  Fission yeast Dna2 is required for generation of the telomeric single-strand overhang.

Authors:  Kazunori Tomita; Tatsuya Kibe; Ho-Young Kang; Yeon-Soo Seo; Masahiro Uritani; Takashi Ushimaru; Masaru Ueno
Journal:  Mol Cell Biol       Date:  2004-11       Impact factor: 4.272

Review 10.  Sex and the single cell: meiosis in yeast.

Authors:  G S Roeder
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-07       Impact factor: 11.205
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