Literature DB >> 8363365

Characterization of the gene for chromosomal trimethoprim-sensitive dihydrofolate reductase of Staphylococcus aureus ATCC 25923.

G E Dale1, R L Then, D Stüber.   

Abstract

The gene for the trimethoprim-sensitive (Tmps) chromosomal dihydrofolate reductase (DHFR) of Staphylococcus aureus ATCC 25923 was cloned and characterized. The structural gene encodes a polypeptide of 159 amino acid residues and has a calculated molecular weight of 18,251. The amino acid sequences of this Tmps DHFR and those of the trimethoprim-resistant type S1 DHFR encoded by transposon Tn4003 are 80% identical. In contrast to the trimethoprim-resistant enzyme, the Tmps DHFR can be highly overexpressed in Escherichia coli, with most of the recombinant protein occurring in a soluble and an active form.

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Year:  1993        PMID： 8363365      PMCID： PMC187982          DOI： 10.1128/AAC.37.7.1400

Source DB:  PubMed          Journal:  Antimicrob Agents Chemother        ISSN： 0066-4804            Impact factor:   5.191


  20 in total

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Review 2.  Antimicrobial resistance of Staphylococcus aureus: genetic basis.

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Journal:  Microbiol Rev       Date:  1987-03

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Authors:  C C Lee; X W Wu; R A Gibbs; R G Cook; D M Muzny; C T Caskey
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4.  N-terminal amino acid sequence of the chromosomal dihydrofolate reductase purified from trimethoprim-resistant Staphylococcus aureus.

Authors:  P G Hartman; M Stähli; H P Kocher; R L Then
Journal:  FEBS Lett       Date:  1988-12-19       Impact factor: 4.124

5.  Localization of Bacillus subtilis sacU(Hy) mutations to two linked genes with similarities to the conserved procaryotic family of two-component signalling systems.

Authors:  D J Henner; M Yang; E Ferrari
Journal:  J Bacteriol       Date:  1988-11       Impact factor: 3.490

6.  A comprehensive set of sequence analysis programs for the VAX.

Authors:  J Devereux; P Haeberli; O Smithies
Journal:  Nucleic Acids Res       Date:  1984-01-11       Impact factor: 16.971

7.  Genomic sequencing.

Authors:  G M Church; W Gilbert
Journal:  Proc Natl Acad Sci U S A       Date:  1984-04       Impact factor: 11.205

8.  Nucleotide sequence of the thymidylate synthase B and dihydrofolate reductase genes contained in one Bacillus subtilis operon.

Authors:  M Iwakura; M Kawata; K Tsuda; T Tanaka
Journal:  Gene       Date:  1988-04-15       Impact factor: 3.688

9.  Trimethoprim resistance transposon Tn4003 from Staphylococcus aureus encodes genes for a dihydrofolate reductase and thymidylate synthetase flanked by three copies of IS257.

Authors:  D A Rouch; L J Messerotti; L S Loo; C A Jackson; R A Skurray
Journal:  Mol Microbiol       Date:  1989-02       Impact factor: 3.501

10.  A novel in vitro transcription-translation system: accurate and efficient synthesis of single proteins from cloned DNA sequences.

Authors:  D Stueber; I Ibrahimi; D Cutler; B Dobberstein; H Bujard
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
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  13 in total

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Journal:  J Clin Microbiol       Date:  2005-10       Impact factor: 5.948

2.  Mutations in the dihydrofolate reductase gene of trimethoprim-resistant isolates of Streptococcus pneumoniae.

Authors:  P V Adrian; K P Klugman
Journal:  Antimicrob Agents Chemother       Date:  1997-11       Impact factor: 5.191

3.  New mobile gene cassettes containing an aminoglycoside resistance gene, aacA7, and a chloramphenicol resistance gene, catB3, in an integron in pBWH301.

Authors:  K L Bunny; R M Hall; H W Stokes
Journal:  Antimicrob Agents Chemother       Date:  1995-03       Impact factor: 5.191

Review 4.  Trimethoprim and sulfonamide resistance.

Authors:  P Huovinen; L Sundström; G Swedberg; O Sköld
Journal:  Antimicrob Agents Chemother       Date:  1995-02       Impact factor: 5.191

5.  Possible role of insertion sequence IS257 in dissemination and expression of high- and low-level trimethoprim resistance in staphylococci.

Authors:  A Leelaporn; N Firth; M E Byrne; E Roper; R A Skurray
Journal:  Antimicrob Agents Chemother       Date:  1994-10       Impact factor: 5.191

6.  Towards the understanding of resistance mechanisms in clinically isolated trimethoprim-resistant, methicillin-resistant Staphylococcus aureus dihydrofolate reductase.

Authors:  Kathleen M Frey; Michael N Lombardo; Dennis L Wright; Amy C Anderson
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7.  In vitro efficacy of new antifolates against trimethoprim-resistant Bacillus anthracis.

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8.  Antistaphylococcal activity of dihydrophthalazine antifolates, a family of novel antibacterial drugs.

Authors:  Catherine Clark; Lois M Ednie; Gengrong Lin; Kathy Smith; Klaudia Kosowska-Shick; Pamela McGhee; Bonifacio Dewasse; Linda Beachel; Patrick Caspers; Bérangère Gaucher; Gürkan Mert; Stuart Shapiro; Peter C Appelbaum
Journal:  Antimicrob Agents Chemother       Date:  2009-02-02       Impact factor: 5.191

9.  Cloning and molecular analysis of the dihydrofolate reductase gene from Lactococcus lactis.

Authors:  K Leszczyńska; A Bolhuis; K Leenhouts; G Venema; P Cegłowski
Journal:  Appl Environ Microbiol       Date:  1995-02       Impact factor: 4.792

10.  Characterization of the gene for the chromosomal dihydrofolate reductase (DHFR) of Staphylococcus epidermidis ATCC 14990: the origin of the trimethoprim-resistant S1 DHFR from Staphylococcus aureus?

Authors:  G E Dale; C Broger; P G Hartman; H Langen; M G Page; R L Then; D Stüber
Journal:  J Bacteriol       Date:  1995-06       Impact factor: 3.490
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