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Literature DB >> 8359169

Detection of toxigenic Clostridium difficile in fecal samples by colony blot hybridization.

M J Wolfhagen¹, A C Fluit, M Jansze, C M Rademaker, J Verhoef.

Abstract

A hybridization assay for detection of toxigenic Clostridium difficile in fecal samples was developed and compared with the classical tissue culture cytotoxicity assay. A DNA fragment probe specific for the toxin B gene of Clostridium difficile was synthesized by the polymerase chain reaction and labelled with digoxigenin. Fecal samples were cultured for 24 hours, replica-plated and hybridized with the probe. The hybridization assay had a sensitivity of 100%, specificity of 96.7%, positive predictive value of 86.7% and negative predictive value of 100% compared with the cytotoxicity assay.

Entities: Chemical Disease Species

Mesh：

Substances：
DNA, Bacterial

Year: 1993 PMID： 8359169 DOI： 10.1007/bf01967444

Source DB: PubMed Journal: Eur J Clin Microbiol Infect Dis ISSN： 0934-9723 Impact factor: 3.267

17 in total

1. Evaluation of a new commercial Clostridium difficile toxin A enzyme immunoassay using diarrhoeal stools.

Authors: M Delmée; T Mackey; A Hamitou
Journal: Eur J Clin Microbiol Infect Dis Date: 1992-03 Impact factor: 3.267

2. Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B.

Authors: A C Fluit; M J Wolfhagen; G P Verdonk; M Jansze; R Torensma; J Verhoef
Journal: J Clin Microbiol Date: 1991-11 Impact factor: 5.948

3. Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A.

Authors: P C De Girolami; P A Hanff; K Eichelberger; L Longhi; H Teresa; J Pratt; A Cheng; J M Letourneau; G M Thorne
Journal: J Clin Microbiol Date: 1992-05 Impact factor: 5.948

4. Molecular screening of Clostridium difficile toxins A and B genetic determinants and identification of mutant strains.

Authors: D E McMillin; L L Muldrow; S J Leggette; Y Abdulahi; U M Ekanemesang
Journal: FEMS Microbiol Lett Date: 1991-02 Impact factor: 2.742

5. Detection of Clostridium difficile toxins A (enterotoxin) and B (cytotoxin) in clinical specimens. Evaluation of a latex agglutination test.

Authors: L R Peterson; J J Holter; C J Shanholtzer; C R Garrett; D N Gerding
Journal: Am J Clin Pathol Date: 1986-08 Impact factor: 2.493

6. Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

Authors: J R DiPersio; F J Varga; D L Conwell; J A Kraft; K J Kozak; D H Willis
Journal: J Clin Microbiol Date: 1991-12 Impact factor: 5.948

Detection of toxigenic Clostridium difficile in fecal samples by colony blot hybridization.

1. Evaluation of a new commercial Clostridium difficile toxin A enzyme immunoassay using diarrhoeal stools.

2. Nontoxigenic strains of Clostridium difficile lack the genes for both toxin A and toxin B.

3. Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A.

4. Molecular screening of Clostridium difficile toxins A and B genetic determinants and identification of mutant strains.

5. Detection of Clostridium difficile toxins A (enterotoxin) and B (cytotoxin) in clinical specimens. Evaluation of a latex agglutination test.

6. Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

7. Use of the polymerase chain reaction for the specific and direct detection of Clostridium difficile in human feces.

8. Characterization of a toxin A-negative, toxin B-positive strain of Clostridium difficile.

9. Characterization of cross-reactive proteins detected by Culturette Brand Rapid Latex Test for Clostridium difficile.

10. Identification of toxigenic Clostridium difficile by the polymerase chain reaction.

1. Rapid detection of toxigenic Clostridium difficile in fecal samples by magnetic immuno PCR assay.

2. Clinical significance of Clostridium difficile and its toxins in faeces of immunocompromised children.