Literature DB >> 3739972

Detection of Clostridium difficile toxins A (enterotoxin) and B (cytotoxin) in clinical specimens. Evaluation of a latex agglutination test.

L R Peterson, J J Holter, C J Shanholtzer, C R Garrett, D N Gerding.   

Abstract

A new latex test, Culturette Brand Rapid Latex Test for detection of Clostridium difficile toxin A, was tested on 408 stool samples. In 247 frozen tissue culture supernate specimens previously obtained from patients with C. difficile-associated diarrhea (CAD), the latex test (enterotoxin) was positive in 182 (74%) as compared with 194 (79%) for the repeat tissue culture (P greater than 0.1) cytotoxin (toxin B) test. Testing of 161 fresh stool samples found the latex test superior to tissue culture (P less than 0.05) in cases of CAD (90% positivity vs. 70%), with the two tests being equal in both non-CAD diarrheal and non-diarrheal control groups. In vitro evaluation of 61 C. difficile isolates found all (100%) to be producers of enterotoxin A, while only 53 (87%) produced toxin B. The latex test for C. difficile toxin detection is a rapid, simple test for use in the diagnosis in CAD.

Entities:  

Mesh：

Substances：

Year:  1986        PMID： 3739972     DOI： 10.1093/ajcp/86.2.208

Source DB:  PubMed          Journal:  Am J Clin Pathol        ISSN： 0002-9173            Impact factor:   2.493


  18 in total

1.  Multicenter evaluation of a new enzyme immunoassay for detection of Clostridium difficile enterotoxin A.

Authors:  P C De Girolami; P A Hanff; K Eichelberger; L Longhi; H Teresa; J Pratt; A Cheng; J M Letourneau; G M Thorne
Journal:  J Clin Microbiol       Date:  1992-05       Impact factor: 5.948

Review 2.  Clostridium difficile: its disease and toxins.

Authors:  D M Lyerly; H C Krivan; T D Wilkins
Journal:  Clin Microbiol Rev       Date:  1988-01       Impact factor: 26.132

3.  Comparison of culture, cytotoxin assay and two EIA tests with clinical diagnosis of Clostridium difficile-associated diarrhea.

Authors:  M Binning; M A John; B C Schieven; T W Austin; R Lannigan; Z Hussain
Journal:  Can J Infect Dis       Date:  1994-07

4.  Multicenter evaluation of four methods for Clostridium difficile detection: ImmunoCard C. difficile, cytotoxin assay, culture, and latex agglutination.

Authors:  J L Staneck; L S Weckbach; S D Allen; J A Siders; P H Gilligan; G Coppitt; J A Kraft; D H Willis
Journal:  J Clin Microbiol       Date:  1996-11       Impact factor: 5.948

5.  Detection of toxigenic Clostridium difficile in fecal samples by colony blot hybridization.

Authors:  M J Wolfhagen; A C Fluit; M Jansze; C M Rademaker; J Verhoef
Journal:  Eur J Clin Microbiol Infect Dis       Date:  1993-06       Impact factor: 3.267

6.  Commercial latex agglutination test for detection of Clostridium difficile-associated diarrhea.

Authors:  M T Kelly; S G Champagne; C H Sherlock; M A Noble; H J Freeman; J A Smith
Journal:  J Clin Microbiol       Date:  1987-07       Impact factor: 5.948

7.  Monospecific antibodies for detection of Clostridium difficile enterotoxin.

Authors:  P Rautenberg; F Stender; U Ullmann
Journal:  Eur J Clin Microbiol       Date:  1987-02       Impact factor: 3.267

8.  Development of a rapid and efficient restriction endonuclease analysis typing system for Clostridium difficile and correlation with other typing systems.

Authors:  C R Clabots; S Johnson; K M Bettin; P A Mathie; M E Mulligan; D R Schaberg; L R Peterson; D N Gerding
Journal:  J Clin Microbiol       Date:  1993-07       Impact factor: 5.948

Review 9.  Clostridium difficile: clinical disease and diagnosis.

Authors:  F C Knoop; M Owens; I C Crocker
Journal:  Clin Microbiol Rev       Date:  1993-07       Impact factor: 26.132

10.  Development of a rapid enzyme immunoassay for Clostridium difficile toxin A and its use in the diagnosis of C. difficile-associated disease.

Authors:  J R DiPersio; F J Varga; D L Conwell; J A Kraft; K J Kozak; D H Willis
Journal:  J Clin Microbiol       Date:  1991-12       Impact factor: 5.948
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.