Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

Literature DB >> 8358830

Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

A E Vainio¹, H T Torkkeli, T Tuusa, S A Aho, B R Fagerström, M P Korhola.

Abstract

A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-acid residues. Comparison with other fungal glucoamylase amino-acid sequences showed homologies of 37-48%. The glucoamylase cDNA, when introduced into Saccharomyces cerevisiae under the control of the yeast ADC1 promoter, directed the secretion of active glucoamylase P into the growth medium.

Entities: Chemical Gene Species

Mesh：

Substances：

Year: 1993 PMID： 8358830 DOI： 10.1007/bf00324663

Source DB: PubMed Journal: Curr Genet ISSN： 0172-8083 Impact factor: 3.886

26 in total

1. Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae.

Authors: V V Joutsjoki
Journal: Curr Genet Date: 1994 Nov-Dec Impact factor: 3.886

Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

1. Construction of a Stable alpha-Galactosidase-Producing Baker's Yeast Strain.

Review 2. Sequences important for gene expression in filamentous fungi.

3. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

4. A simple and very efficient method for generating cDNA libraries.

5. Patterns of amino acids near signal-sequence cleavage sites.

6. Nucleotide sequence of the glucoamylase gene GLU1 in the yeast Saccharomycopsis fibuligera.

7. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.

8. DNA sequence required for efficient transcription termination in yeast.

9. Studies on transformation of Escherichia coli with plasmids.

10. Nucleotide sequence of the extracellular glucoamylase gene STA1 in the yeast Saccharomyces diastaticus.

1. Construction by one-step gene replacement of Trichoderma reesei strains that produce the glucoamylase P of Hormoconis resinae.

2. Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae.

3. Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae.

4. Cloning of Corticium rolfsii glucoamylase cDNA and its expression in Saccharomyces cerevisiae.