Literature DB >> 8358825

Detection of gene-disruption events in Aspergillus transformants by polymerase chain reaction direct from conidiospores.

A Aufauvre-Brown1, C M Tang, D W Holden.   

Abstract

We describe a rapid method for the identification of gene disruption events after DNA-mediated transformation of Aspergillus fumigatus. This involves a polymerase chain reaction in which the target DNA is added in the form of intact conidiospores. Using one primer specific to the plasmid DNA and a second primer specific to the target gene on the chromosome, it is possible to identify gene disruption events among the more common ectopic integrations approximately 4 h after sporulating transformants appear on selective medium.

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Year:  1993        PMID： 8358825     DOI： 10.1007/bf00324683

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  6 in total

1.  Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.

Authors:  R Rothstein
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  Use of polymerase chain reaction for rapid detection of gene insertions in whole yeast cells.

Authors:  G M Sathe; S O'Brien; M M McLaughlin; F Watson; G P Livi
Journal:  Nucleic Acids Res       Date:  1991-09-11       Impact factor: 16.971

3.  Recombinant fragment assay for gene targetting based on the polymerase chain reaction.

Authors:  H S Kim; O Smithies
Journal:  Nucleic Acids Res       Date:  1988-09-26       Impact factor: 16.971

4.  Secretion of a potential virulence factor, a fungal ribonucleotoxin, during human aspergillosis infections.

Authors:  B Lamy; M Moutaouakil; J P Latge; J Davies
Journal:  Mol Microbiol       Date:  1991-07       Impact factor: 3.501

5.  An Aspergillus fumigatus alkaline protease mutant constructed by gene disruption is deficient in extracellular elastase activity.

Authors:  C M Tang; J Cohen; D W Holden
Journal:  Mol Microbiol       Date:  1992-06       Impact factor: 3.501

Review 6.  Transformation in fungi.

Authors:  J R Fincham
Journal:  Microbiol Rev       Date:  1989-03
  6 in total
  5 in total

1.  Agrobacterium-mediated insertional mutagenesis (AIM) of the entomopathogenic fungus Beauveria bassiana.

Authors:  Andreas Leclerque; Hong Wan; Anette Abschütz; Siwei Chen; Galina V Mitina; Gisbert Zimmermann; Hans Ulrich Schairer
Journal:  Curr Genet       Date:  2003-11-22       Impact factor: 3.886

2.  Development of an oligonucleotide probe for Aureobasidium pullulans based on the small-subunit rRNA gene.

Authors:  S Li; D Cullen; M Hjort; R Spear; J H Andrews
Journal:  Appl Environ Microbiol       Date:  1996-05       Impact factor: 4.792

3.  Molecular Diagnosis of Pathogenic Sporothrix Species.

Authors:  Anderson Messias Rodrigues; G Sybren de Hoog; Zoilo Pires de Camargo
Journal:  PLoS Negl Trop Dis       Date:  2015-12-01

4.  Species-specific PCR to describe local-scale distributions of four cryptic species in the Penicillium chrysogenum complex.

Authors:  Alexander G P Browne; Matthew C Fisher; Daniel A Henk
Journal:  Fungal Ecol       Date:  2013-10       Impact factor: 3.404

5.  The Aspergillus PacC zinc finger transcription factor mediates regulation of both acid- and alkaline-expressed genes by ambient pH.

Authors:  J Tilburn; S Sarkar; D A Widdick; E A Espeso; M Orejas; J Mungroo; M A Peñalva; H N Arst
Journal:  EMBO J       Date:  1995-02-15       Impact factor: 11.598
  5 in total

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