Literature DB >> 8354232

Application of high-performance capillary electrophoresis to the analysis of H1 histones.

H Lindner1, M Wurm, A Dirschlmayer, B Sarg, W Helliger.   

Abstract

High-performance capillary electrophoresis for the separation of rat testis H1 histone variants and their phosphorylated modifications is described. The influence of buffer pH, hydroxypropylmethyl cellulose, and buffer concentration has been investigated. Under optimized conditions (500 mM phosphate buffer, pH 2, 0.03% hydroxypropylmethyl cellulose) using an uncoated capillary, eight H1 histone subfractions, including two H1(0) histones and H1t and their phosphorylated modifications, are resolved. Application of capillary electrophoresis to the separation of H1 histones provides an important new alternative to high-performance liquid chromatography (HPLC) and traditional gel electrophoresis.

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Year:  1993        PMID： 8354232     DOI： 10.1002/elps.1150140174

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  7 in total

Review 1.  The significance, development and progress of high-throughput combinatorial histone code analysis.

Authors:  Nicolas L Young; Peter A Dimaggio; Benjamin A Garcia
Journal:  Cell Mol Life Sci       Date:  2010-08-04       Impact factor: 9.261

2.  Immunohistochemical demonstration of histone H1(0) in human breast carcinoma.

Authors:  Nora N Kostova; Ljuba N Srebreva; Angel D Milev; Olga G Bogdanova; Ingemar Rundquist; Herbert H Lindner; Dimiter V Markov
Journal:  Histochem Cell Biol       Date:  2005-11-03       Impact factor: 4.304

Review 3.  Mass spectrometry-based strategies for characterization of histones and their post-translational modifications.

Authors:  Xiaodan Su; Chen Ren; Michael A Freitas
Journal:  Expert Rev Proteomics       Date:  2007-04       Impact factor: 3.940

4.  Comparing and combining capillary electrophoresis electrospray ionization mass spectrometry and nano-liquid chromatography electrospray ionization mass spectrometry for the characterization of post-translationally modified histones.

Authors:  Bettina Sarg; Klaus Faserl; Leopold Kremser; Bernhard Halfinger; Roberto Sebastiano; Herbert H Lindner
Journal:  Mol Cell Proteomics       Date:  2013-05-29       Impact factor: 5.911

5.  Changes in the protein pattern of H1 histones associated with apoptotic DNA fragmentation.

Authors:  M Kratzmeier; W Albig; T Meergans; D Doenecke
Journal:  Biochem J       Date:  1999-01-15       Impact factor: 3.857

6.  Histone H1 interphase phosphorylation becomes largely established in G1 or early S phase and differs in G1 between T-lymphoblastoid cells and normal T cells.

Authors:  Anna Gréen; Bettina Sarg; Henrik Gréen; Anita Lönn; Herbert H Lindner; Ingemar Rundquist
Journal:  Epigenetics Chromatin       Date:  2011-08-05       Impact factor: 4.954

7.  Quantitative proteomics using ultralow flow capillary electrophoresis-mass spectrometry.

Authors:  Klaus Faserl; Leopold Kremser; Martin Müller; David Teis; Herbert H Lindner
Journal:  Anal Chem       Date:  2015-04-16       Impact factor: 6.986
  7 in total

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