Literature DB >> 8349709

The anticodon and discriminator base are important for aminoacylation of Escherichia coli tRNA(Asn).

S Li1, H Pelka, L H Schulman.   

Abstract

A gel shift assay that distinguishes the aminoacylated form from the deacylated form of tRNAs was used to study the requirements for aminoacylation of Escherichia coli tRNA(Asn) in vivo. tRNA(Asn) derivatives containing single base changes in their anticodons or discriminator bases were constructed, and the extent of in vivo aminoacylation was determined directly. Substitution of U35 with C35 or U36 with C36 abolished aminoacylation of the tRNA. Substitution of G34 with C34 converted tRNA(Asn) into a lysine acceptor. Thus, each of the anticodon nucleotides are important for aminoacylation of tRNA(Asn). Substitution of discriminator base G73 with A73 affected the extent of aminoacylation in vivo indicating that the discriminator base also contributes to aminoacylation of tRNA(Asn).

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Year:  1993        PMID： 8349709

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  9 in total

1.  An extensive study of mutation and selection on the wobble nucleotide in tRNA anticodons in fungal mitochondrial genomes.

Authors:  Malisa Carullo; Xuhua Xia
Journal:  J Mol Evol       Date:  2008-04-10       Impact factor: 2.395

2.  Genetic selection for active E.coli amber tRNA(Asn) exclusively led to glutamine inserting suppressors.

Authors:  F Martin; G Eriani; J Reinbolt; G Dirheimer; J Gangloff
Journal:  Nucleic Acids Res       Date:  1995-03-11       Impact factor: 16.971

3.  Quantification of the Abundance and Charging Levels of Transfer RNAs in Escherichia coli.

Authors:  Thomas Søndergaard Stenum; Michael A Sørensen; Sine Lo Svenningsen
Journal:  J Vis Exp       Date:  2017-08-22       Impact factor: 1.355

4.  Non-discriminating and discriminating aspartyl-tRNA synthetases differ in the anticodon-binding domain.

Authors:  Christophe Charron; Hervé Roy; Mickael Blaise; Richard Giegé; Daniel Kern
Journal:  EMBO J       Date:  2003-04-01       Impact factor: 11.598

5.  The crystal structures of T. thermophilus lysyl-tRNA synthetase complexed with E. coli tRNA(Lys) and a T. thermophilus tRNA(Lys) transcript: anticodon recognition and conformational changes upon binding of a lysyl-adenylate analogue.

Authors:  S Cusack; A Yaremchuk; M Tukalo
Journal:  EMBO J       Date:  1996-11-15       Impact factor: 11.598

Review 6.  Biochemistry of Aminoacyl tRNA Synthetase and tRNAs and Their Engineering for Cell-Free and Synthetic Cell Applications.

Authors:  Ragunathan Bava Ganesh; Sebastian J Maerkl
Journal:  Front Bioeng Biotechnol       Date:  2022-07-01

7.  Genome of bacteriophage P1.

Authors:  Małgorzata B Łobocka; Debra J Rose; Guy Plunkett; Marek Rusin; Arkadiusz Samojedny; Hansjörg Lehnherr; Michael B Yarmolinsky; Frederick R Blattner
Journal:  J Bacteriol       Date:  2004-11       Impact factor: 3.490

8.  Aminoacylation and conformational properties of yeast mitochondrial tRNA mutants with respiratory deficiency.

Authors:  Silvia Francisci; Cristina DE Luca; Romina Oliva; Veronica Morea; Anna Tramontano; Laura Frontali
Journal:  RNA       Date:  2005-06       Impact factor: 4.942

9.  The exchange of the discriminator base A73 for G is alone sufficient to convert human tRNA(Leu) into a serine-acceptor in vitro.

Authors:  K Breitschopf; H J Gross
Journal:  EMBO J       Date:  1994-07-01       Impact factor: 11.598
  9 in total

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