Literature DB >> 8341592

The role of the preserved sequences of Dam methylase.

J B Guyot1, J Grassi, U Hahn, W Guschlbauer.   

Abstract

We have undertaken a site directed mutational analysis of two of the preserved regions in the amino acid sequence of Dam methylase in order to characterize their role. Mutations in region IV (sequence DPPY) abolish catalytic activity and greatly affect AdoMet crosslinking. Mutants in region III display a lowered specific activity with an unchanged AdoMet crosslinking capacity. We have also made a series of deletions both at the N and C terminal parts of the protein, which have been found to provide inactive enzyme. We discuss the significance of these results for the understanding of the functional properties of the enzyme.

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Year:  1993        PMID： 8341592      PMCID： PMC309753          DOI： 10.1093/nar/21.14.3183

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  43 in total

1.  The E. coli cell surface specifically prevents the initiation of DNA replication at oriC on hemimethylated DNA templates.

Authors:  A Landoulsi; A Malki; R Kern; M Kohiyama; P Hughes
Journal:  Cell       Date:  1990-11-30       Impact factor: 41.582

2.  A general method for rapid site-directed mutagenesis using the polymerase chain reaction.

Authors:  O Landt; H P Grunert; U Hahn
Journal:  Gene       Date:  1990-11-30       Impact factor: 3.688

3.  Nucleotide sequence of the FokI restriction-modification system: separate strand-specificity domains in the methyltransferase.

Authors:  M C Looney; L S Moran; W E Jack; G R Feehery; J S Benner; B E Slatko; G G Wilson
Journal:  Gene       Date:  1989-08-15       Impact factor: 3.688

4.  Evolution of type II DNA methyltransferases. A gene duplication model.

Authors:  R Lauster
Journal:  J Mol Biol       Date:  1989-03-20       Impact factor: 5.469

5.  The DNA and S-adenosylmethionine-binding regions of EcoDam and related methyltransferases.

Authors:  W Guschlbauer
Journal:  Gene       Date:  1988-12-25       Impact factor: 3.688

6.  The double role of methyl donor and allosteric effector of S-adenosyl-methionine for Dam methylase of E. coli.

Authors:  A Bergerat; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1990-08-11       Impact factor: 16.971

7.  Primary structure of a DNA (N6-adenine)-methyltransferase from Escherichia coli virus T1. DNA sequence, genomic organization, and comparative analysis.

Authors:  E Schneider-Scherzer; B Auer; E J de Groot; M Schweiger
Journal:  J Biol Chem       Date:  1990-04-15       Impact factor: 5.157

8.  Single amino acid changes that alter the DNA sequence specificity of the DNA-[N6-adenine] methyltransferase (Dam) of bacteriophage T4.

Authors:  Z Miner; S L Schlagman; S Hattman
Journal:  Nucleic Acids Res       Date:  1989-10-25       Impact factor: 16.971

Review 9.  The great GATC: DNA methylation in E. coli.

Authors:  F Barras; M G Marinus
Journal:  Trends Genet       Date:  1989-05       Impact factor: 11.639

10.  Inhibition of EcoRI DNA methylase with cofactor analogs.

Authors:  N O Reich; N Mashhoon
Journal:  J Biol Chem       Date:  1990-05-25       Impact factor: 5.157
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  12 in total

1.  DNA binding properties in vivo and target recognition domain sequence alignment analyses of wild-type and mutant RsrI [N6-adenine] DNA methyltransferases.

Authors:  S S Szegedi; R I Gumport
Journal:  Nucleic Acids Res       Date:  2000-10-15       Impact factor: 16.971

2.  Substrate DNA and cofactor regulate the activities of a multi-functional restriction-modification enzyme, BcgI.

Authors:  H Kong; C L Smith
Journal:  Nucleic Acids Res       Date:  1997-09-15       Impact factor: 16.971

3.  Mutations within the catalytic motif of DNA adenine methyltransferase (Dam) of Aeromonas hydrophila cause the virulence of the Dam-overproducing strain to revert to that of the wild-type phenotype.

Authors:  Tatiana E Erova; Amin A Fadl; Jian Sha; Bijay K Khajanchi; Lakshmi L Pillai; Elena V Kozlova; Ashok K Chopra
Journal:  Infect Immun       Date:  2006-10       Impact factor: 3.441

Review 4.  Structure, function and mechanism of exocyclic DNA methyltransferases.

Authors:  Shivakumara Bheemanaik; Yeturu V R Reddy; Desirazu N Rao
Journal:  Biochem J       Date:  2006-10-15       Impact factor: 3.857

5.  Identification of the m6Am Methyltransferase PCIF1 Reveals the Location and Functions of m6Am in the Transcriptome.

Authors:  Konstantinos Boulias; Diana Toczydłowska-Socha; Ben R Hawley; Noa Liberman; Ken Takashima; Sara Zaccara; Théo Guez; Jean-Jacques Vasseur; Françoise Debart; L Aravind; Samie R Jaffrey; Eric Lieberman Greer
Journal:  Mol Cell       Date:  2019-07-03       Impact factor: 17.970

6.  Dam methylase from Escherichia coli: kinetic studies using modified DNA oligomers: hemimethylated substrates.

Authors:  S Marzabal; S DuBois; V Thielking; A Cano; R Eritja; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1995-09-25       Impact factor: 16.971

7.  Structural basis for binding of RNA and cofactor by a KsgA methyltransferase.

Authors:  Chao Tu; Joseph E Tropea; Brian P Austin; Donald L Court; David S Waugh; Xinhua Ji
Journal:  Structure       Date:  2009-03-11       Impact factor: 5.006

8.  Dam methyltransferase from Escherichia coli: sequence of a peptide segment involved in S-adenosyl-methionine binding.

Authors:  C Wenzel; W Guschlbauer
Journal:  Nucleic Acids Res       Date:  1993-09-25       Impact factor: 16.971

9.  DNA methylation impacts gene expression and ensures hypoxic survival of Mycobacterium tuberculosis.

Authors:  Scarlet S Shell; Erin G Prestwich; Seung-Hun Baek; Rupal R Shah; Christopher M Sassetti; Peter C Dedon; Sarah M Fortune
Journal:  PLoS Pathog       Date:  2013-07-04       Impact factor: 6.823

10.  A mutational analysis of the two motifs common to adenine methyltransferases.

Authors:  D F Willcock; D T Dryden; N E Murray
Journal:  EMBO J       Date:  1994-08-15       Impact factor: 11.598
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