| Literature DB >> 832918 |
A A Yunis, G K Arimura, D J Russin.
Abstract
An undifferentiated human pancreatic carcinoma has been established in continuous culture and is grown in Dulbecco's modified. Eagle's medium fortified with 10% fetal calf serum and 2.5% horse serum. The established cell line (MIA PaCa-2) has a doubling time of 40 h. The cells are large with abundant cytoplasm, exhibit a high degree of aneuploidy and have a tendency to grow on top of other cells. MIA PaCa-2 grows in soft agar with a colony-forming efficiency of 19%. Both MIA PaCa-2 cells and a cell line from another pancreatic carcinoma obtained from National Cancer Institute (NCI) are sensitive to asparaginase, a property not shared by several other human tumor cell lines tested.Entities:
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Year: 1977 PMID： 832918 DOI： 10.1002/ijc.2910190118
Source DB: PubMed Journal: Int J Cancer ISSN： 0020-7136 Impact factor: 7.396