Literature DB >> 830657

Temperature-sensitive mutations affecting flagellar assembly and function in Chlamydomonas reinhardtii.

B Huang, M R Rifkin, D J Luck.   

Abstract

A series of conditional mutants of the algal, biflagellate Chlamydomonas reinhardtii with temperature-sensitive defects in flagellar assembly and function were isolated. The genetics and phenotypes of 21 mutants displaying a rapid alteration in flagellar function upon shift from the permissive (20 degrees C) to the restrictive (32 degrees C) temperatures are described. These mutants designated as "drop-down" or dd-mutants have been placed in four categories on the basis of their defective phenotypes: (a) dd-assembly mutants - the preformed flagella are resorbed at 32 degrees C and reassembly of flagella is inhibited; (b) dd-fragile flagella mutants - the flagella are lost by detachment at 32 degrees C, but can be reassembled; (c) dd-motility mutants - the flagella are retained at 32 degrees C, but are functionally defective; (d) dd-lethal mutants - display combined defects in flagellar function and cell growth. Tetrad analysis of the mutants back-crossed to wild-type, recombination analysis of intermutant crosses, and complementation tests in the construction of heterozygous diploid strains indicate that at least 14 nuclear genetic loci are represented among 21 mutants. The availability of temperature-sensitive mutations affecting the assembly and function of the flagellum suggests that the morphogenesis of this complex eukaryotic organelle is amenable to genetic dissection.

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Year:  1977        PMID: 830657      PMCID: PMC2110986          DOI: 10.1083/jcb.72.1.67

Source DB:  PubMed          Journal:  J Cell Biol        ISSN: 0021-9525            Impact factor:   10.539


  10 in total

1.  A genetic analysis of linkage group I of Chlamydomonas reinhardi.

Authors:  W T EBERSOLD; R P LEVINE
Journal:  Z Vererbungsl       Date:  1959

2.  A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.

Authors:  K BURTON
Journal:  Biochem J       Date:  1956-02       Impact factor: 3.857

3.  Nutritional studies with Chlamydomonas reinhardi.

Authors:  R SAGER; S GRANICK
Journal:  Ann N Y Acad Sci       Date:  1953-10-14       Impact factor: 5.691

4.  Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.

Authors:  D S Gorman; R P Levine
Journal:  Proc Natl Acad Sci U S A       Date:  1965-12       Impact factor: 11.205

5.  The flagellar apparatus as a model organelle for the study of growth and morphopoiesis. With an appendix. Temperature control apparatus used in flagellar regeneration experiments.

Authors:  J Randall; H R Munden; P H Prest
Journal:  Proc R Soc Lond B Biol Sci       Date:  1969-04-15

6.  Induction of Mendelian and non-Mendelian streptomycin resistant mutants during the synchronous cell cycle of Chlamydomonas reinhardtii.

Authors:  R W Lee; R F Jones
Journal:  Mol Gen Genet       Date:  1973-03-01

7.  [Autolysis of the cell wall of gametes of Chlamydomonas reinhardii].

Authors:  H Claes
Journal:  Arch Mikrobiol       Date:  1971

8.  Fine structure of cell division in Chlamydomonas reinhardi. Basal bodies and microtubules.

Authors:  U G Johnson; K R Porter
Journal:  J Cell Biol       Date:  1968-08       Impact factor: 10.539

9.  BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii.

Authors:  U W Goodenough; H S StClair
Journal:  J Cell Biol       Date:  1975-09       Impact factor: 10.539

10.  The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.

Authors:  E S REYNOLDS
Journal:  J Cell Biol       Date:  1963-04       Impact factor: 10.539

  10 in total
  55 in total

1.  Total internal reflection fluorescence (TIRF) microscopy of Chlamydomonas flagella.

Authors:  Benjamin D Engel; Karl-Ferdinand Lechtreck; Tsuyoshi Sakai; Mitsuo Ikebe; George B Witman; Wallace F Marshall
Journal:  Methods Cell Biol       Date:  2009-12-04       Impact factor: 1.441

Review 2.  Mechanism of ciliary disassembly.

Authors:  Yinwen Liang; Dan Meng; Bing Zhu; Junmin Pan
Journal:  Cell Mol Life Sci       Date:  2016-02-11       Impact factor: 9.261

3.  Genetic and genomic approaches to identify genes involved in flagellar assembly in Chlamydomonas reinhardtii.

Authors:  Huawen Lin; Susan K Dutcher
Journal:  Methods Cell Biol       Date:  2015-02-14       Impact factor: 1.441

4.  Genetic analysis of long-flagella mutants of Chlamydomonas reinhardtii.

Authors:  S E Barsel; D E Wexler; P A Lefebvre
Journal:  Genetics       Date:  1988-04       Impact factor: 4.562

5.  The FLA3 KAP subunit is required for localization of kinesin-2 to the site of flagellar assembly and processive anterograde intraflagellar transport.

Authors:  Joshua Mueller; Catherine A Perrone; Raqual Bower; Douglas G Cole; Mary E Porter
Journal:  Mol Biol Cell       Date:  2004-12-22       Impact factor: 4.138

6.  Kinesin-3 KLP-6 regulates intraflagellar transport in male-specific cilia of Caenorhabditis elegans.

Authors:  Natalia S Morsci; Maureen M Barr
Journal:  Curr Biol       Date:  2011-07-14       Impact factor: 10.834

Review 7.  The Intraflagellar Transport Machinery.

Authors:  Michael Taschner; Esben Lorentzen
Journal:  Cold Spring Harb Perspect Biol       Date:  2016-10-03       Impact factor: 10.005

8.  Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons.

Authors:  D G Cole; D R Diener; A L Himelblau; P L Beech; J C Fuster; J L Rosenbaum
Journal:  J Cell Biol       Date:  1998-05-18       Impact factor: 10.539

9.  Mechanoreception in motile flagella of Chlamydomonas.

Authors:  Kenta Fujiu; Yoshitaka Nakayama; Hidetoshi Iida; Masahiro Sokabe; Kenjiro Yoshimura
Journal:  Nat Cell Biol       Date:  2011-04-10       Impact factor: 28.824

10.  Intraflagellar transport particle size scales inversely with flagellar length: revisiting the balance-point length control model.

Authors:  Benjamin D Engel; William B Ludington; Wallace F Marshall
Journal:  J Cell Biol       Date:  2009-10-05       Impact factor: 10.539

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