OBJECTIVE: A large population of F2 rats, obtained from a cross between male Lyon hypertensive (LH) rats and female Lyon normotensive (LN) rats, was studied in order to assess the relationship between increased body weight, hyperlipidaemia and high blood pressure which characterize LH rats. METHODS: Mean arterial pressure (MAP) was recorded in male, conscious, freely moving LH, LN, F1 and F2 rats aged 30 weeks. Plasma total cholesterol, high-density lipoprotein-, low-density lipoprotein- and very low-density lipoprotein-cholesterol, phospholipids, triglycerides, insulin and glucose were measured. RESULTS: In the F2 cohort it was observed that high MAP was a recessive trait that depends on several genes and was unrelated to body weight. The left ventricular weight, corrected for tibia length, was correlated with MAP. Plasma total and high-density lipoprotein-cholesterol and phospholipids concentrations were lower in the F1 rats than in the LN rats, suggesting an overdominance of the LN alleles. In the F2 rats MAP was related to total, high-density lipoprotein- and low-density lipoprotein-cholesterol. Plasma triglycerides, insulin and the insulin:glucose ratio, which were higher in the LH rats than in the LN rats, were also correlated with MAP in the F2 cohort. Using stepwise multiple regression analysis, MAP remained correlated with plasma total cholesterol, insulin and the insulin:glucose ratio, but not with triglycerides. CONCLUSIONS: Hypertension in LH rats is a recessive trait that is independent of body weight. In addition, the cosegregation of blood pressure with plasma cholesterol and, to a lesser degree, with insulin levels, which was observed in the present study provides the first direct evidence that these phenotypes are associated and are not due simply to genetic drift in the Lyon model.
OBJECTIVE: A large population of F2 rats, obtained from a cross between male Lyon hypertensive (LH) rats and female Lyon normotensive (LN) rats, was studied in order to assess the relationship between increased body weight, hyperlipidaemia and high blood pressure which characterize LH rats. METHODS: Mean arterial pressure (MAP) was recorded in male, conscious, freely moving LH, LN, F1 and F2 rats aged 30 weeks. Plasma total cholesterol, high-density lipoprotein-, low-density lipoprotein- and very low-density lipoprotein-cholesterol, phospholipids, triglycerides, insulin and glucose were measured. RESULTS: In the F2 cohort it was observed that high MAP was a recessive trait that depends on several genes and was unrelated to body weight. The left ventricular weight, corrected for tibia length, was correlated with MAP. Plasma total and high-density lipoprotein-cholesterol and phospholipids concentrations were lower in the F1 rats than in the LN rats, suggesting an overdominance of the LN alleles. In the F2 rats MAP was related to total, high-density lipoprotein- and low-density lipoprotein-cholesterol. Plasma triglycerides, insulin and the insulin:glucose ratio, which were higher in the LH rats than in the LN rats, were also correlated with MAP in the F2 cohort. Using stepwise multiple regression analysis, MAP remained correlated with plasma total cholesterol, insulin and the insulin:glucose ratio, but not with triglycerides. CONCLUSIONS:Hypertension in LH rats is a recessive trait that is independent of body weight. In addition, the cosegregation of blood pressure with plasma cholesterol and, to a lesser degree, with insulin levels, which was observed in the present study provides the first direct evidence that these phenotypes are associated and are not due simply to genetic drift in the Lyon model.
Authors: A Bottger; H A van Lith; V Kren; D Krenová; V Bílá; J Vorlícek; V Zídek; A Musilová; M Zdobinská; J M Wang; B F van Zutphen; T W Kurtz; M Pravenec Journal: J Clin Invest Date: 1996-08-01 Impact factor: 14.808
Authors: Jinkai Wang; Man Chun John Ma; Amanda K Mennie; Janette M Pettus; Yang Xu; Lan Lin; Matthew G Traxler; Jessica Jakoubek; Santosh S Atanur; Timothy J Aitman; Yi Xing; Anne E Kwitek Journal: Circ Cardiovasc Genet Date: 2015-01-08
Authors: Francine Z Marques; Priscilla R Prestes; Leonardo B Pinheiro; Katrina Scurrah; Kerry R Emslie; Maciej Tomaszewski; Stephen B Harrap; Fadi J Charchar Journal: BMC Med Genomics Date: 2014-07-15 Impact factor: 3.063
Authors: David Martín-Gálvez; Denis Dunoyer de Segonzac; Man Chun John Ma; Anne E Kwitek; David Thybert; Paul Flicek Journal: BMC Genomics Date: 2017-12-22 Impact factor: 3.969
Authors: Man Chun John Ma; Janette M Pettus; Jessica A Jakoubek; Matthew G Traxler; Karen C Clark; Amanda K Mennie; Anne E Kwitek Journal: PLoS One Date: 2017-08-08 Impact factor: 3.240