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Literature DB >> 82935

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

J Tomassini, R Roychoudhury, R Wu, R J Roberts.

Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

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Year: 1978 PMID： 82935 PMCID： PMC342733 DOI： 10.1093/nar/5.11.4055

Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971

13 in total

1. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).

Authors: I R LEHMAN; A L NUSSBAUM
Journal: J Biol Chem Date: 1964-08 Impact factor: 5.157

2. Nucleotide recognition sequence at the cleavage site of Haemophilus aegyptius II (Hae II) restriction endonuclease.

Authors: C D Tu; R Roychoudhury; R Wu
Journal: Biochem Biophys Res Commun Date: 1976-09-07 Impact factor: 3.575

3. Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

Authors: C P Bahl; R Wu; J Stawinsky; S A Narang
Journal: Proc Natl Acad Sci U S A Date: 1977-03 Impact factor: 11.205

4. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

Authors: P H Seeburg; J Shine; J A Martial; J D Baxter; H M Goodman
Journal: Nature Date: 1977-12-08 Impact factor: 49.962

5. Synthetic polynucleotides. Ther terminal addition of riboadenylic acid to deoxyoligonucleotides by terminal deoxynucleotidyl transferase as a tool for the specific labelling of deoxyoligonucleotides at the 3'-ends.

Authors: H Kössel; R Roychoudhury
Journal: Eur J Biochem Date: 1971-09-24

6. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

Authors: D I Smith; F R Blattner; J Davies
Journal: Nucleic Acids Res Date: 1976-02 Impact factor: 16.971

Review 7. Restriction endonucleases.

Authors: R J Roberts
Journal: CRC Crit Rev Biochem Date: 1976-11

8. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

Authors: E Jay; R Bambara; R Padmanabhan; R Wu
Journal: Nucleic Acids Res Date: 1974-03 Impact factor: 16.971

9. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

Authors: R Roychoudhury; E Jay; R Wu
Journal: Nucleic Acids Res Date: 1976-04 Impact factor: 16.971

10. Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.

Authors: D Brutlag; K Fry; T Nelson; P Hung
Journal: Cell Date: 1977-03 Impact factor: 41.582

17 in total

1. Segments of simian virus 40 DNA spanning most of the leader sequence of the major late viral messenger RNA are dispensable.

Authors: K N Subramanian
Journal: Proc Natl Acad Sci U S A Date: 1979-06 Impact factor: 11.205

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae.

1. THE DEOXYRIBONUCLEASES OF ESCHERICHIA COLI. V. ON THE SPECIFICITY OF EXONUCLEASE I (PHOSPHODIESTERASE).

2. Nucleotide recognition sequence at the cleavage site of Haemophilus aegyptius II (Hae II) restriction endonuclease.

3. Minimal length of the lactose operator sequence for the specific recognition by the lactose repressor.

4. Nucleotide sequence and amplification in bacteria of structural gene for rat growth hormone.

5. Synthetic polynucleotides. Ther terminal addition of riboadenylic acid to deoxyoligonucleotides by terminal deoxynucleotidyl transferase as a tool for the specific labelling of deoxyoligonucleotides at the 3'-ends.

6. The isolation and partial characterization of a new restriction endonuclease from Providencia stuartii.

Review 7. Restriction endonucleases.

8. DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.

9. Terminal labeling and addition of homopolymer tracts to duplex DNA fragments by terminal deoxynucleotidyl transferase.

10. Synthesis of hybrid bacterial plasmids containing highly repeated satellite DNA.

1. Segments of simian virus 40 DNA spanning most of the leader sequence of the major late viral messenger RNA are dispensable.

2. M.KpnI is an adenine-methyltransferase.

3. Type II restriction endonuclease R.KpnI is a member of the HNH nuclease superfamily.

4. Genetic organization of the KpnI restriction--modification system.

5. Restriction enzymes and their isoschizomers.

6. Restriction enzymes and their isoschizomers.

7. Restriction and modification enzymes and their recognition sequences.

8. Restriction enzymes and their isoschizomers.

Review 9. Restriction and modification enzymes and their recognition sequences.

10. Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases.