| Literature DB >> 8290552 |
S J Libby1, W Goebel, A Ludwig, N Buchmeier, F Bowe, F C Fang, D G Guiney, J G Songer, F Heffron.
Abstract
A Salmonella gene encoding a cytolysin has been identified by screening for hemolysis on blood agar. DNA sequence analyses together with genetic mapping in Salmonella suggest that it is unrelated to other toxins or hemolysins. The gene (slyA) is present in every strain of Salmonella examined, in Shigella, and in enteroinvasive Escherichia coli but not in other Enterobacteriaceae. SlyA (salmolysin) purified from a derivative of the original clone has hemolytic and cytolytic activity and has a molecular weight predicted by the DNA sequence. The median lethal dose and infection kinetics in mice suggest that the toxin is required for virulence and facilitates Salmonella survival within mouse peritoneal macrophages.Entities:
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Year: 1994 PMID： 8290552 PMCID： PMC42974 DOI： 10.1073/pnas.91.2.489
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205