Literature DB >> 8285672

Purification and characterization of two 1,4-beta-xylan endohydrolases from the rumen fungus Neocallimastix frontalis.

B Gomez de Segura1, M Fevre.   

Abstract

Two beta-endoxylanases produced by Neocallimastix frontalis have been purified by ammonium sulfate precipitation, gel filtration, and ion-exchange chromatography. Xylanase I is a nonglycosylated protein with an apparent molecular mass of 45 kDa. Xylanase II is a glycoprotein with an apparent molecular mass of 70 kDa. The pH optima of these enzymes were 5.5 and 6, respectively, and the temperature optimum was 55 degrees C for each enzyme. The endo mode of action of the enzymes was revealed by thin-layer chromatography of xylan hydrolysates. Antibodies raised against each purified protein exhibited no cross-reaction, confirming the biochemical specificities of the enzymes. Both enzymes exhibited carboxymethyl cellulase activity, and xylanase I was absorbed on crystalline cellulose, indicating that these enzymes might belong to the F family of beta-1,4-glycanases.

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Year:  1993        PMID： 8285672      PMCID： PMC182512          DOI： 10.1128/aem.59.11.3654-3660.1993

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  A novel polysaccharide hydrolase cDNA (celD) from Neocallimastix patriciarum encoding three multi-functional catalytic domains with high endoglucanase, cellobiohydrolase and xylanase activities.

Authors:  G P Xue; K S Gobius; C G Orpin
Journal:  J Gen Microbiol       Date:  1992-11

Review 2.  Domains in microbial beta-1, 4-glycanases: sequence conservation, function, and enzyme families.

Authors:  N R Gilkes; B Henrissat; D G Kilburn; R C Miller; R A Warren
Journal:  Microbiol Rev       Date:  1991-06

3.  Purification and Characterization of an Aspecific Glycoside Hydrolase from the Anaerobic Ruminal Fungus Neocallimastix frontalis.

Authors:  M Hebraud; M Fevre
Journal:  Appl Environ Microbiol       Date:  1990-10       Impact factor: 4.792

4.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

Authors:  M M Bradford
Journal:  Anal Biochem       Date:  1976-05-07       Impact factor: 3.365

5.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors:  H Towbin; T Staehelin; J Gordon
Journal:  Proc Natl Acad Sci U S A       Date:  1979-09       Impact factor: 11.205

6.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

7.  Purification and characterization of an extracellular beta-xylosidase from the rumen anaerobic fungus Neocallimastix frontalis.

Authors:  M Hebraud; M Fevre
Journal:  FEMS Microbiol Lett       Date:  1990-10       Impact factor: 2.742

8.  Cellulase families revealed by hydrophobic cluster analysis.

Authors:  B Henrissat; M Claeyssens; P Tomme; L Lemesle; J P Mornon
Journal:  Gene       Date:  1989-09-01       Impact factor: 3.688

9.  Structural and functional relationships in two families of beta-1,4-glycanases.

Authors:  N R Gilkes; M Claeyssens; R Aebersold; B Henrissat; A Meinke; H D Morrison; D G Kilburn; R A Warren; R C Miller
Journal:  Eur J Biochem       Date:  1991-12-05

10.  Characterization of N-linked oligosaccharides by affinoblotting with concanavalin A-peroxidase and treatment of the blots with glycosidases.

Authors:  L Faye; M J Chrispeels
Journal:  Anal Biochem       Date:  1985-08-15       Impact factor: 3.365
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  6 in total

Review 1.  A new look at xylanases: an overview of purification strategies.

Authors:  Paula Sá-Pereira; Helena Paveia; Maria Costa-Ferreira; Maria Aires-Barros
Journal:  Mol Biotechnol       Date:  2003-07       Impact factor: 2.695

2.  Location by fluorescence microscopy of glycosidases and a xylanase in the anaerobic gut fungi Caecomyces communis, Neocallimastix frontalis, and Piromyces rhizinflata.

Authors:  A Breton; B Gaillard-Martinie; C Gerbi; B Gomez de Ségura; R Durand; B Kherratia
Journal:  Curr Microbiol       Date:  1995-10       Impact factor: 2.188

3.  Effects of live Saccharomyces cerevisiae cells on zoospore germination, growth, and cellulolytic activity of the rumen anaerobic fungus, Neocallimastix frontalis MCH3.

Authors:  F Chaucheyras; G Fonty; G Bertin; P Gouet
Journal:  Curr Microbiol       Date:  1995-10       Impact factor: 2.188

4.  Characterization of the "promoter region" of the enolase-encoding gene enol from the anaerobic fungus Neocallimastix frontalis: sequence and promoter analysis.

Authors:  M Fischer; R Durand; M Fèvre
Journal:  Curr Genet       Date:  1995-06       Impact factor: 3.886

5.  Functional characterization of cellulases identified from the cow rumen fungus Neocallimastix patriciarum W5 by transcriptomic and secretomic analyses.

Authors:  Tzi-Yuan Wang; Hsin-Liang Chen; Mei-Yeh J Lu; Yo-Chia Chen; Huang-Mo Sung; Chi-Tang Mao; Hsing-Yi Cho; Huei-Mien Ke; Teh-Yang Hwa; Sz-Kai Ruan; Kuo-Yen Hung; Chih-Kuan Chen; Jeng-Yi Li; Yueh-Chin Wu; Yu-Hsiang Chen; Shao-Pei Chou; Ya-Wen Tsai; Te-Chin Chu; Chun-Chieh A Shih; Wen-Hsiung Li; Ming-Che Shih
Journal:  Biotechnol Biofuels       Date:  2011-08-17       Impact factor: 6.040

Review 6.  Nucleotide and protein researches on anaerobic fungi during four decades.

Authors:  Jongsoo Chang; Hyunjin Park
Journal:  J Anim Sci Technol       Date:  2020-03-31
  6 in total

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