Literature DB >> 8283968

Cloning and analysis of the human myocardial Na+/H+ exchanger.

L Fliegel1, J R Dyck, H Wang, C Fong, R S Haworth.   

Abstract

The Na+/H+ exchanger is an integral membrane protein that is universally distribute in mammalian tissues and is responsible for intracellular pH regulation. Several isoforms of the Na+/H+ exchanger exist (NHE-1-NHE-4). The first that was cloned is the amiloride sensitive isoform (NHE-1). Using a fragment of the rabbit cardiac Na+/H+ exchanger cDNA clone we isolated and sequenced Na+/H+ exchanger cDNA from a human heart coding for the complete human Na+/H+ exchanger (NHE-1 isoform). Two overlapping cDNA clones were obtained, giving a combined sequence that contained both 3' and 5' untranslated regions. The 5' and 3' untranslated regions proved to be highly homologous to human sequences described earlier but contained some variations that could affect the mRNA stability and/or the efficiency of translation of the Na+/H+ exchanger. Northern blot analysis and reverse transcriptase polymerase chain reaction confirmed the presence of the 5 kb NHE-1 message in primary cultures of isolated myocytes.

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Year:  1993        PMID： 8283968     DOI： 10.1007/BF00936442

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  16 in total

1.  Molecular cloning of putative members of the Na/H exchanger gene family. cDNA cloning, deduced amino acid sequence, and mRNA tissue expression of the rat Na/H exchanger NHE-1 and two structurally related proteins.

Authors:  J Orlowski; R A Kandasamy; G E Shull
Journal:  J Biol Chem       Date:  1992-05-05       Impact factor: 5.157

2.  Identification of the protein and cDNA of the cardiac Na+/H+ exchanger.

Authors:  L Fliegel; C Sardet; J Pouyssegur; A Barr
Journal:  FEBS Lett       Date:  1991-02-11       Impact factor: 4.124

3.  Growth factors induce phosphorylation of the Na+/H+ antiporter, glycoprotein of 110 kD.

Authors:  C Sardet; L Counillon; A Franchi; J Pouysségur
Journal:  Science       Date:  1990-02-09       Impact factor: 47.728

4.  Molecular cloning, primary structure, and expression of the human growth factor-activatable Na+/H+ antiporter.

Authors:  C Sardet; A Franchi; J Pouysségur
Journal:  Cell       Date:  1989-01-27       Impact factor: 41.582

Review 5.  Mechanisms of regulation of the Na+/H+ exchanger.

Authors:  S Grinstein; A Rothstein
Journal:  J Membr Biol       Date:  1986       Impact factor: 1.843

6.  Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.

Authors:  R K Saiki; D H Gelfand; S Stoffel; S J Scharf; R Higuchi; G T Horn; K B Mullis; H A Erlich
Journal:  Science       Date:  1988-01-29       Impact factor: 47.728

7.  Pharmacologically different Na/H antiporters on the apical and basolateral surfaces of cultured porcine kidney cells (LLC-PK1).

Authors:  J G Haggerty; N Agarwal; R F Reilly; E A Adelberg; C W Slayman
Journal:  Proc Natl Acad Sci U S A       Date:  1988-09       Impact factor: 11.205

8.  Characterization of the placental brush border membrane Na+/H+ exchanger: identification of thiol-dependent transitions in apparent molecular size.

Authors:  L Fliegel; R S Haworth; J R Dyck
Journal:  Biochem J       Date:  1993-01-01       Impact factor: 3.857

9.  Cloning, sequencing, and expression of Na(+)-H+ antiporter cDNAs from human tissues.

Authors:  K Takaichi; D Wang; D F Balkovetz; D G Warnock
Journal:  Am J Physiol       Date:  1992-04

10.  Distal cis-acting promoter sequences mediate glucocorticoid stimulation of cardiac atrial natriuretic factor gene transcription.

Authors:  S Argentin; Y L Sun; I Lihrmann; T J Schmidt; J Drouin; M Nemer
Journal:  J Biol Chem       Date:  1991-12-05       Impact factor: 5.157
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  12 in total

Review 1.  Functional and cellular regulation of the myocardial Na+/H+ exchanger.

Authors:  L Fliegel
Journal:  J Thromb Thrombolysis       Date:  1999-07       Impact factor: 2.300

2.  Elevated expression of activated Na(+)/H(+) exchanger protein induces hypertrophy in isolated rat neonatal ventricular cardiomyocytes.

Authors:  Fatima Mraiche; Larry Fliegel
Journal:  Mol Cell Biochem       Date:  2011-07-01       Impact factor: 3.396

3.  Gender-specific effects of exercise on cardiac pathology in Na(+)/H(+) exchanger overexpressing mice.

Authors:  Heather Vandertol Vanier; Fatima Mraiche; Xiuju Li; Larry Fliegel
Journal:  Mol Cell Biochem       Date:  2012-05-25       Impact factor: 3.396

Review 4.  Na+-H+ exchanger-1 (NHE1) regulation in kidney proximal tubule.

Authors:  Mark D Parker; Evan J Myers; Jeffrey R Schelling
Journal:  Cell Mol Life Sci       Date:  2015-02-14       Impact factor: 9.261

5.  Ras triggers acidosis-induced activation of the extracellular-signal-regulated kinase pathway in cardiac myocytes.

Authors:  Robert S Haworth; Semjidmaa Dashnyam; Metin Avkiran
Journal:  Biochem J       Date:  2006-11-01       Impact factor: 3.857

6.  Structural study of the porcine Na+/H+ exchanger NHE1 gene and its 5'-flanking region.

Authors:  A L Façanha; M C dos Reis; M Montero-Lomeli
Journal:  Mol Cell Biochem       Date:  2000-07       Impact factor: 3.396

7.  Effect of Hoe 694, a novel Na(+)-H+ exchange inhibitor, on intracellular pH regulation in the guinea-pig ventricular myocyte.

Authors:  S H Loh; B Sun; R D Vaughan-Jones
Journal:  Br J Pharmacol       Date:  1996-08       Impact factor: 8.739

8.  Structure and analysis of the mouse Na+/H+ exchanger (NHE1) gene: homology and conservation of splice sites.

Authors:  H Wang; D Singh; W Yang; J R Dyck; L Fliegel
Journal:  Mol Cell Biochem       Date:  1996-12-20       Impact factor: 3.396

Review 9.  Role of Genetic Mutations of the Na+/H+ Exchanger Isoform 1, in Human Disease and Protein Targeting and Activity.

Authors:  Larry Fliegel
Journal:  Mol Cell Biochem       Date:  2020-11-17       Impact factor: 3.396

10.  Amiloride and guggulsterone suppression of esophageal cancer cell growth in vitro and in nude mouse xenografts.

Authors:  Baoxiang Guan; Ashraful Hoque; Xiaochun Xu
Journal:  Front Biol (Beijing)       Date:  2014-02
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