Literature DB >> 8276879

Characterization and crystallization of recombinant human neurotrophin-4.

J P Fandl1, N J Tobkes, N Q McDonald, W A Hendrickson, T E Ryan, S Nigam, A Acheson, H Cudny, N Panayotatos.   

Abstract

Neurotrophin-4 (NT-4) is the most recently discovered member of the neurotrophin family. We have expressed, refolded, and purified recombinant human NT-4 from Escherichia coli and compared it with recombinant human NT-4 secreted into the culture medium of baculovirus-infected insect cells. Both preparations were characterized and determined to be indistinguishable according to several biochemical criteria. Recombinant NT-4 from E. coli was crystallized in a form suitable for x-ray analysis, and characterization of these crystals indicated that NT-4 was present as a dimer within the asymmetric unit. NT-4 was active in promoting the survival of rat TrkB receptor-expressing fibroblasts, but was inactive on embryonic chicken sensory neurons, unlike the other members of the neurotrophin family and in contrast to the reported activities of partially purified NT-4.

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Year:  1994        PMID： 8276879

Source DB:  PubMed          Journal:  J Biol Chem        ISSN： 0021-9258            Impact factor:   5.157


  8 in total

Review 1.  Neurotrophins: roles in neuronal development and function.

Authors:  E J Huang; L F Reichardt
Journal:  Annu Rev Neurosci       Date:  2001       Impact factor: 12.449

2.  Crystals of the neurotrophins.

Authors:  R C Robinson; C Radziejewski; D I Stuart; E Y Jones
Journal:  Protein Sci       Date:  1996-05       Impact factor: 6.725

Review 3.  Neurotrophin-4: the odd one out in the neurotrophin family.

Authors:  C F Ibáñez
Journal:  Neurochem Res       Date:  1996-07       Impact factor: 3.996

4.  Directed evolution of brain-derived neurotrophic factor for improved folding and expression in Saccharomyces cerevisiae.

Authors:  Michael L Burns; Thomas M Malott; Kevin J Metcalf; Benjamin J Hackel; Jonah R Chan; Eric V Shusta
Journal:  Appl Environ Microbiol       Date:  2014-07-11       Impact factor: 4.792

5.  Circular dichroism and crosslinking studies of the interaction between four neurotrophins and the extracellular domain of the low-affinity neurotrophin receptor.

Authors:  D E Timm; A H Ross; K E Neet
Journal:  Protein Sci       Date:  1994-03       Impact factor: 6.725

6.  Pan-neurotrophin 1: a genetically engineered neurotrophic factor displaying multiple specificities in peripheral neurons in vitro and in vivo.

Authors:  L L Ilag; R Curtis; D Glass; H Funakoshi; N J Tobkes; T E Ryan; A Acheson; R M Lindsay; H Persson; G D Yancopoulos
Journal:  Proc Natl Acad Sci U S A       Date:  1995-01-17       Impact factor: 11.205

7.  Crystallization and preliminary structural studies of neurotrophin-3.

Authors:  J A Kelly; E Singer; T D Osslund; T O Yeates
Journal:  Protein Sci       Date:  1994-06       Impact factor: 6.725

8.  Functional analysis of mutant neurotrophins deficient in low-affinity binding reveals a role for p75LNGFR in NT-4 signalling.

Authors:  M Rydén; J Murray-Rust; D Glass; L L Ilag; M Trupp; G D Yancopoulos; N Q McDonald; C F Ibáñez
Journal:  EMBO J       Date:  1995-05-01       Impact factor: 11.598
  8 in total

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