Literature DB >> 8276108

Replication of adenovirus DNA in vitro is ATP-independent.

R Pronk1, W Van Driel, P C Van der Vliet.   

Abstract

Using a reconstituted system for adenovirus DNA replication we tested the requirements for ATP and divalent cations. At the standard Mg2+ concentration ATP stimulated initiation 5 to 10-fold. However, this effect was caused by complexing Mg2+. At the optimal Mg2+ concentration ATP was not required for initiation or elongation. Besides Mg2+ also Mn2+, Ca2+ and Ba2+ were shown to support initiation whereas for elongation only Mg2+ was accepted. Since Mn2+ could efficiently be used for DNA chain elongation on synthetic templates we hypothesize that Mg2+ is essential for the transition of initiation to elongation.

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Year:  1994        PMID： 8276108     DOI： 10.1016/0014-5793(94)80624-1

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  7 in total

1.  Identification of an RNA hairpin in poliovirus RNA that serves as the primary template in the in vitro uridylylation of VPg.

Authors:  A V Paul; E Rieder; D W Kim; J H van Boom; E Wimmer
Journal:  J Virol       Date:  2000-11       Impact factor: 5.103

2.  Multimerization of the adenovirus DNA-binding protein is the driving force for ATP-independent DNA unwinding during strand displacement synthesis.

Authors:  J Dekker; P N Kanellopoulos; A K Loonstra; J A van Oosterhout; K Leonard; P A Tucker; P C van der Vliet
Journal:  EMBO J       Date:  1997-03-17       Impact factor: 11.598

3.  Adenovirus DNA polymerase: domain organisation and interaction with preterminal protein.

Authors:  E J Parker; C H Botting; A Webster; R T Hay
Journal:  Nucleic Acids Res       Date:  1998-03-01       Impact factor: 16.971

4.  Biochemical and genetic studies of the initiation of human rhinovirus 2 RNA replication: purification and enzymatic analysis of the RNA-dependent RNA polymerase 3D(pol).

Authors:  K Gerber; E Wimmer; A V Paul
Journal:  J Virol       Date:  2001-11       Impact factor: 5.103

5.  The adenovirus priming protein pTP contributes to the kinetics of initiation of DNA replication.

Authors:  Monika E Mysiak; P Elly Holthuizen; Peter C van der Vliet
Journal:  Nucleic Acids Res       Date:  2004-07-25       Impact factor: 16.971

6.  Biochemical and genetic studies of the VPg uridylylation reaction catalyzed by the RNA polymerase of poliovirus.

Authors:  Aniko V Paul; Julia Peters; JoAnn Mugavero; Jiang Yin; Jacques H van Boom; E Wimmer
Journal:  J Virol       Date:  2003-01       Impact factor: 5.103

7.  A precursor terminal protein-trinucleotide intermediate during initiation of adenovirus DNA replication: regeneration of molecular ends in vitro by a jumping back mechanism.

Authors:  A J King; P C van der Vliet
Journal:  EMBO J       Date:  1994-12-01       Impact factor: 11.598
  7 in total

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