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Literature DB >> 8270257

Isolation and characterization of the human genomic locus coding for the putative metastasis control gene nm23-H1.

S Dooley¹, T Seib, M Engel, B Theisinger, H Janz, K Piontek, K D Zang, C Welter.

Abstract

Nm23-H1 gene expression is inversely correlated with tumor metastatic potential in certain tumors, including melanomas, breast carcinomas, and hepatocellular carcinomas. Using nm23-H1 c-DNA primer and genomic polymerase chain reaction (PCR) amplification, we purified three PCR fragments (one of 4kb and two of 2 kb) covering the whole human genomic locus of the gene (8.460bp). We recombined the PCR products into pUC18 and produced a restriction map to perform subcloning. Complete sequencing of genomic PCR fragments, including the whole coding region of nm23-H1, revealed that the gene consists of five exons and four introns spanning 8.5kb. A sequence homology analysis between human nm23-H1 and the homolog gene of the rat (NDP-K beta) shows that exon-intron boundaries are well conserved between these two species.

Entities: Disease Gene Species

Mesh：

Substances：

Year: 1994 PMID： 8270257 DOI： 10.1007/bf00218915

Source DB: PubMed Journal: Hum Genet ISSN： 0340-6717 Impact factor: 4.132

22 in total

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Authors: R Müllenbach; P J Lagoda; C Welter
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Authors: P S Steeg; K H Cohn; A Leone
Journal: Cancer Cells Date: 1991-07

3. A second form (beta isoform) of nucleoside diphosphate kinase from rat. Isolation and characterization of complementary and genomic DNA and expression.

Authors: N Shimada; N Ishikawa; Y Munakata; T Toda; K Watanabe; N Kimura
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4. Somatic allelic deletion of nm23 in human cancer.

Authors: A Leone; O W McBride; A Weston; M G Wang; P Anglard; C S Cropp; J R Goepel; R Lidereau; R Callahan; W M Linehan
Journal: Cancer Res Date: 1991-05-01 Impact factor: 12.701

5. A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphate kinase.

Authors: J Biggs; E Hersperger; P S Steeg; L A Liotta; A Shearn
Journal: Cell Date: 1990-11-30 Impact factor: 41.582

6. Association of nm23-H1 allelic deletions with distant metastases in colorectal carcinoma.

Authors: K H Cohn; F S Wang; F Desoto-LaPaix; W B Solomon; L G Patterson; M R Arnold; J Weimar; J G Feldman; A T Levy; A Leone
Journal: Lancet Date: 1991-09-21 Impact factor: 79.321

7. Isolation and characterization of a gene encoding rat nucleoside diphosphate kinase.

Authors: N Ishikawa; N Shimada; Y Munakata; K Watanabe; N Kimura
Journal: J Biol Chem Date: 1992-07-15 Impact factor: 5.157

8. Evidence for nm23 RNA overexpression, DNA amplification and mutation in aggressive childhood neuroblastomas.

Authors: A Leone; R C Seeger; C M Hong; Y Y Hu; M J Arboleda; G M Brodeur; D Stram; D J Slamon; P S Steeg
Journal: Oncogene Date: 1993-04 Impact factor: 9.867

9. Identification of a second human nm23 gene, nm23-H2.

Authors: J A Stahl; A Leone; A M Rosengard; L Porter; C R King; P S Steeg
Journal: Cancer Res Date: 1991-01-01 Impact factor: 12.701

10. High levels of nm23-H1 and nm23-H2 messenger RNA in human squamous-cell lung carcinoma are associated with poor differentiation and advanced tumor stages.

Authors: M Engel; B Theisinger; T Seib; G Seitz; H Huwer; K D Zang; C Welter; S Dooley
Journal: Int J Cancer Date: 1993-09-30 Impact factor: 7.396

4 in total