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Literature DB >> 8268784

Setting up a PCR laboratory.

C W Dieffenbach¹, G S Dveksler.

Abstract

Mesh：

Year: 1993 PMID： 8268784 DOI： 10.1101/gr.3.2.s2

Source DB: PubMed Journal: PCR Methods Appl ISSN： 1054-9803

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7 in total

1. Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter.

Authors: María M Peñas; Goretti Azparren; Angel Domínguez; Hans Sommer; Lucía Ramírez; Antonio G Pisabarro
Journal: Mol Genet Genomics Date: 2005-08-17 Impact factor: 3.291

2. Single-tube single-enzyme reverse transcriptase PCR assay for detection of bovine viral diarrhea virus in pooled bovine serum.

Authors: D Weinstock; B Bhudevi; A E Castro
Journal: J Clin Microbiol Date: 2001-01 Impact factor: 5.948

3. Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.

Authors: Hirokazu Takahashi; Hiroyuki Yamazaki; Satoshi Akanuma; Hiroko Kanahara; Toshiyuki Saito; Tomoyuki Chimuro; Takayoshi Kobayashi; Toshio Ohtani; Kimiko Yamamoto; Shigeru Sugiyama; Toshiro Kobori
Journal: PLoS One Date: 2014-02-05 Impact factor: 3.240

Setting up a PCR laboratory.

1. Identification and functional characterisation of ctr1, a Pleurotus ostreatus gene coding for a copper transporter.

2. Single-tube single-enzyme reverse transcriptase PCR assay for detection of bovine viral diarrhea virus in pooled bovine serum.

3. Preparation of Phi29 DNA polymerase free of amplifiable DNA using ethidium monoazide, an ultraviolet-free light-emitting diode lamp and trehalose.

4. Assessment of Capacity to Capture DNA Aerosols by Clean Filters for Molecular Biology Experiments.

5. A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement.

6. A simple modification of PCR thermal profile applied to evade persisting contamination.

Review 7. Issues and prospects of microRNA-based biomarkers in blood and other body fluids.